XU Ai-li, DONG Yu-juan, JIANG Jie-yi, et al. UPLC Fingerprint Analysis and Chemical Compounds Identification of Polygoni Multiflori Radix Formula Granules[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(2): 47-50.
DOI:
XU Ai-li, DONG Yu-juan, JIANG Jie-yi, et al. UPLC Fingerprint Analysis and Chemical Compounds Identification of Polygoni Multiflori Radix Formula Granules[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(2): 47-50. DOI: 10.13422/j.cnki.syfjx.2016020047.
UPLC Fingerprint Analysis and Chemical Compounds Identification of Polygoni Multiflori Radix Formula Granules
Objective:To establish a method of fingerprint analysis on Polygoni Multiflori Radix formula granules by UPLC
identify the main chemical compounds
and provide a scientific method for rapid quality evaluation of Polygoni Multiflori Radix formula granules. Method:Ultra Performance Liquid Chromatography (UPLC) was used on the Agilent Eclipse Plus C18 RRHD (2.1 mm×100 mm
1.8μm)
with acetonitrile-water as the mobile phase for gradient elution. The detective wavelength was set at 254 nm;the flow rate was 0.4 mL·min-1 and the column temperature was maintained at 30℃.Meanwhile
time of flight mass spectroscopy was used to analyze the main chemical compounds in fingerprints. Result:UPLC fingerprints were developed for Polygoni Multiflori Radix formula granules. 12 common peaks were determined
and 5 of them were identified. The similarity was higher than 0.9 between the fingerprints of the different samples of Polygoni Multiflori Radix formula granules and the control fingerprints. Conclusion:The method with good reproducibility is simple
rapid and accurate
which can be used to rapidly and effectively evaluate the quality of Polygoni Multiflori Radix formula granules.
Spectrum-effect Relationship of Bupleuri Radix Processed with Trionyx sinensis Blood for Yin Deficiency Based on Saponins
Analysis on Advantages of New Integration Processing Method in Producing Area of Polygoni Multiflori Radix Praeparata
HPLC Fingerprint Analysis and Multi-component Determination of Substance Benchmark of Yiweitang
UPLC Fingerprint of Notopterygh Rhizoma et Radix Pieces with Different Packaging and Storage Treatments
Stoichiometric Analysis in Fingerprint of Aqueous Extract of Citri Reticulatae Pericarpium and Spectrum-effect Relationship with Diabetic Cognitive Dysfunction
Related Author
HOU Mengyu
ZHAO Xia
GUO Zhiyu
LIU Ting
MA Yuexing
YE Yaohui
XIN Xueying
JING Jialin
Related Institution
Nanchang Medical College
Jiangxi University of Chinese Medicine
Jiangxi Jingde Traditional Chinese Medicine Co. Ltd.
School of Pharmacy, Jiangxi University of Chinese Medicine
Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences