Simultaneous Determination of Four Pharmacodynamic Constituents in Angeitides Mixture Ⅰby HPLC-DAD

ZENG Wei-xin; LIU Li-bin; DUAN Song-leng; JIN Rui; WU Bin; YANG Li; SUN Lu-lu

doi:10.13422/j.cnki.syfjx.2016020055

您当前的位置：

首页 >

文章列表页 >

Simultaneous Determination of Four Pharmacodynamic Constituents in Angeitides Mixture Ⅰby HPLC-DAD

更新时间：2024-01-04

- Simultaneous Determination of Four Pharmacodynamic Constituents in Angeitides Mixture Ⅰby HPLC-DAD
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 22, Issue 2, Pages: 55-58(2016)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2016020055
  CLC： R284.1
- Published：2016
- 稿件说明：
移动端阅览
ZENG Wei-xin, LIU Li-bin, DUAN Song-leng, et al. Simultaneous Determination of Four Pharmacodynamic Constituents in Angeitides Mixture Ⅰby HPLC-DAD[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(2): 55-58.
DOI：

ZENG Wei-xin, LIU Li-bin, DUAN Song-leng, et al. Simultaneous Determination of Four Pharmacodynamic Constituents in Angeitides Mixture Ⅰby HPLC-DAD[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(2): 55-58. DOI： 10.13422/j.cnki.syfjx.2016020055.

摘要

目的:建立HPLC同时测定脉管炎合剂Ⅰ号中咖啡酸、芍药苷、黄芩苷、蒙花苷4种主要药效成分含量的方法。方法:采用Agilent Extend-C18色谱柱(4.6 mm×150 mm

5μm)

流动相乙腈-0.5%磷酸水溶液梯度洗脱

流速1 mL·min-1

柱温25℃

咖啡酸、芍药苷、黄芩苷、蒙花苷的检测波长分别为为323

230

280

334 nm。结果:咖啡酸、芍药苷、黄芩苷、蒙花苷的线性范围分别为0.0448~0.224μg(r=0.9998)

0.5328~2.664μg(r=0.9998)

0.6784~3.392μg(r=0.9999)

0.1000~0.5000μg (r=0.9998);平均加样回收率分别为100.67%(RSD 1.7%)

99.36%(RSD 0.9%)

101.50%(RSD 1.4%)

99.05%(RSD 1.1%)。结论:建立的HPLC方法简便、快速、可靠、重复性好

为脉管炎合剂Ⅰ号的质量控制提供了参考。

Abstract

Objective:Establishment of HPLC-DAD determination of 4 major pharmacodynamic constituents of the mixture of coffee acid

paeoniflorin

baicalin

buddleoside

and the content of the main components of Angeitides mixture I. Method:The Agilent extend-C18 column (4.6 mm×150 mm

5μm) was used

the mobile phase was acetonitrile-0.5% aqueous solution of phosphoric acid with gradient elution at a flow rate of 1 mL·min-1.The column temperature was kept at 25℃ and the detection wavelength of the coffee acid

paeoniflorin

baicalin

buddleoside was 323

230

280

334 nm. Result:The calibration curves of caffeic acid

paeoniflorin

baicalinand buddleoside were linear in the range of 0.0448-0.224(r=0.9999)

0.5328-2.664μg(r=0.9998)

0.6784-3.392μg(r=0.9999)

0.1000-0.5000μg(r=0.9998) and the average recoveries were 100.62%(RSD 1.7%)

99.36%(RSD 0.9%)

101.50%(RSD 1.4%)

99.05%(RSD 1.1%)

respectively. Conclusion:The HPLC method is simple

fast

reliable and reproducible

providing a reference for quality control Angeitides mixture I.

关键词

Keywords

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Atomization Characteristics of Baicalin， Ursodeoxycholic Acid and Caffeic Acid in Tanreqing Inhalation Solution

Determination of Baicalin and Paeoniflorin in Yangkun Pills by HPLC

Optimization of Extraction Technology for Chaiqin Qinggan Formular with Multiple Components Evaluation

Simultaneous Determination of Five Components in Tangminlingwan by HPLC

Related Author

Shao-yong LIU

Xiao-li ZHANG

Mu-sheng ZHANG

Meng-jiao LIU

Chen ZANG

Guo-hua WANG

LI Ming

CHEN Zhi-wei

Related Institution

Shanghai Kaibao Pharmaceutical Co.Ltd.

Institute of Chinese Materia Medica，China Academy of Chinese Medical Sciences

Research Center for Differentiation and Development of Traditional Chinese Medicine （TCM） Basic Theory， Jiangxi Province Key Laboratory of TCM Etiopathogenisis， Jiangxi University of Chinese Medicine

The Affiliated Cancer Hospital of Zhengzhou University/Henan Cancer Hospital

Fuwai Central China Cardiovascular Hospital/Henan Provincial People's Hospital

AI问答

Postal code：100700
Tel：010-84076882 Email：syfjx_2010@188.com
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备11006657号-10 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰