LI Yong, LI Cheng-wang, ZHANG Jie. Simultaneous Determination of Three Active Ingredients in Wanbei Zhike Pill by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(2): 63-66.
DOI:
LI Yong, LI Cheng-wang, ZHANG Jie. Simultaneous Determination of Three Active Ingredients in Wanbei Zhike Pill by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(2): 63-66. DOI: 10.13422/j.cnki.syfjx.2016020063.
Simultaneous Determination of Three Active Ingredients in Wanbei Zhike Pill by HPLC
目的:建立HPLC同时测定皖贝止咳滴丸中贝母素甲、贝母素乙及异贝母素甲含量的方法。方法:采用CAPCELL PAK C18色谱柱
柱温35℃
流动相乙腈-0.1%三乙胺溶液(70:30)
流速1.0 mL·min-1;ELSD参数为漂移管温度95℃
载气流速2.2 mL·min-1。结果:贝母素甲、贝母素乙及异贝母素甲分别在34.33~343.3
30.17~301.7
82.11~821.1 mg·L-1线性关系良好;贝母素甲的平均加样回收率为97.55%
RSD 1.3%
贝母素乙的平均加样回收率为97.99%
RSD 1.8%
异贝母素甲的平均加样回收率为98.42%
RSD 1.4%。结论:该法简便、准确、可靠
可作为皖贝止咳滴丸质量控制的方法。
Abstract
Objective:To establish a method for the simultaneous determination of peimine
peiminine and iso-peimine in Wanbei Zhike pill by HPLC. Method:The analysis was carried out on a CAPCELL PAK C18 column
with column temperature of 35℃;the mobile phase was acetonitrile-0.1% triethylamine solution (70:30) with a flow rate of 1.0 mL·min-1;ELSD parameters:the drift tube temperature was set at 95℃ and the nitrogen flow rate was 2.2 mL·min-1. Result:Peimine
peiminine and iso-peimine had good linear relationship respectively at the range of 34.33-343.3
30.17-301.7
82.11-821.1 mg·L-1;the average recovery rate for peimine
peiminine and iso-peimine was 97.55%
97.99% and 98.42% with RSD of 1.3%
1.8% and 1.4% respectively. Conclusion:The method is simple
accurate
reliable
and can be used as a method for quality control in Wanbei Zhike pill.