Optimization of Vacuum-assisted Extraction Technology for Carthami Flos by Response Surface Methodology

WANG Ya-qi; HE Xiao-ru; WU Zhen-feng; ZHANG Xiao-fei; LAN Ji-ping; ZHENG Qin; YANG Ming

doi:10.13422/j.cnki.syfjx.2016030005

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Optimization of Vacuum-assisted Extraction Technology for Carthami Flos by Response Surface Methodology

更新时间：2024-01-04

- Optimization of Vacuum-assisted Extraction Technology for Carthami Flos by Response Surface Methodology
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 22, Issue 3, Pages: 5-8(2016)
- 作者机构：
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- DOI：10.13422/j.cnki.syfjx.2016030005
  CLC： R284.2
- Published：2016
- 稿件说明：
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WANG Ya-qi, HE Xiao-ru, WU Zhen-feng, et al. Optimization of Vacuum-assisted Extraction Technology for Carthami Flos by Response Surface Methodology[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(3): 5-8.
DOI：

WANG Ya-qi, HE Xiao-ru, WU Zhen-feng, et al. Optimization of Vacuum-assisted Extraction Technology for Carthami Flos by Response Surface Methodology[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(3): 5-8. DOI： 10.13422/j.cnki.syfjx.2016030005.

摘要

目的:优化红花减压辅助提取的工艺参数

为该工艺的推广与应用提供参考。方法:以羟基红花黄色素A得率为指标

采用响应面分析法对乙醇体积分数、沸腾温度、提取次数、提取时间等参数进行考察

结合扫描电镜微观分析不同处理方式药材表面的微观形态。结果:最佳提取工艺为加20倍量50%乙醇于65℃提取2次

每次42 min。羟基红花黄色素A得率1.51%。结论:优选的减压提取工艺稳定可靠

羟基红花黄色素A得率显著高于传统回流提取法

提示减压提取法适合中药材中热敏性成分的提取。

Abstract

Objective: To investigate rationality of vacuum-assisted extraction (VAE) technology for Carthami Flos and optimize its extraction conditions. Method: Response surface methodology was used to optimize VAE technology of Carthami Flos with yield of hydroxy safflower yellow A as index

its major factors were boiling temperature

ethanol concentration

extraction time and cycles

which had influence on yield of hydroxy safflower yellow A were tested by an Box-Behnken design.Besides

micro-morphology of plant materials were observed by scanning electron microscope (SEM). Result: Optimum extraction conditions were as follows:extracted twice with 20 times the amount of 50% ethanol at 65℃

extraction time of 42 min for per time.Under these conditions

yield of hydroxy safflower yellow A reached 1.51%. Conclusion: Compared with conventional heat reflux extraction

VAE has higher extraction efficiency.VAE is an efficient and simple method for extracting hydroxy safflower yellow A from Carthami Flos

which shows great potential for becoming an alternative technique for extraction of heat sensitive compounds from traditional Chinese medicine.

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