WANG Li-hong, XIE Bao-xian, XIN Yi-zhou, et al. Chemical Compositions of Mahuang Tincture[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(3): 40-43.
DOI:
WANG Li-hong, XIE Bao-xian, XIN Yi-zhou, et al. Chemical Compositions of Mahuang Tincture[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(3): 40-43. DOI: 10.13422/j.cnki.syfjx.2016030040.
Objective: To investigate the chemical compositions of Mahuang tincture formula. Method: Compounds were isolated and purified from the formula with silica gel column chromatography
ODS reversed-phase column chromatography and recrystallization
and their structures were identified on the basis of spectral analyses and physicochemical properties. Result: Twelve compounds were isolated from the ethylacetate extract (with strongest anti-inflammatory activity) of Mahuang tincture and their structures were identified as berberine (1)
columbamine (2)
coptisine (3)
8-oxo-epiberberine (4)
8-oxo-coptisine (5)
geniposide (6)
brucine (7)
strychnine (8)
vomicine (9)
α-amyrin (10)
β-sitosterol (11)
daucosterol (12). Conclusion: All 12 compounds were isolated from this formula for the first time and alkaloids were the main compositions
which must be the material basis for pharmacological activities of Mahuang tincture.