Objective: To establish a method for the content determination of gastrodin

4-hydroxybenzyi aicohol

ferulic acid and ligustilide in extracts of Ligusticumc chuanxiong-Gastrodia elata. Method: The determination was performed on Inertsil ODS-3 chromatographic column (4.6 mm×250 mm

5 μm) with mobile phase of methanol-0.05% phosphoric acid for gradient elute

at the flow rate of 1.0 mL·min-1. The detection wavelength was set at 220 nm (gastrodin

4-hydroxybenzyi aicohol)

320 nm (ferulic acid

ligustilide) and column temperature was 25℃. Result: The four compounds were well separated and showed good linearity within the concentration ranges tested. Their average recovery rate was 104.0%

103.4%

100.6% and 101.8% for gastrodin

4-hydroxybenzyi aicohol

ferulic acid and ligustilide;and RSD of the above four components was 0.4%

0.5%

4.5% and 2.6%

respectively. Conclusion: This method is simple

precise and repeatable

and it can be used to determine the four active components in Ligusticumc chuanxiong-Gastrodia elata.