YUAN Hui-jun, WANG Lan, CHENG Long, et al. Pharmacokinetic Analysis of Tanshinone Ⅱ and Salvianolic Acid B from Yindan Xinnaotong Soft Capsules in Rat Plasma[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(3): 76-80.
DOI:
YUAN Hui-jun, WANG Lan, CHENG Long, et al. Pharmacokinetic Analysis of Tanshinone Ⅱ and Salvianolic Acid B from Yindan Xinnaotong Soft Capsules in Rat Plasma[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(3): 76-80. DOI: 10.13422/j.cnki.syfjx.2016030076.
Pharmacokinetic Analysis of Tanshinone Ⅱ and Salvianolic Acid B from Yindan Xinnaotong Soft Capsules in Rat Plasma
reliable and sensitive method of UPLC-MS/MS with an atmospheric-pressure chemical ionization(APCI) source for determination of tanshinone ⅡA and salvianolic acid B in rat plasma after oral administration of Yindan Xinnaotong soft capsules. Method: UPLC-MS/MS was employed to measure plasma concentration at different time after ig administration of Yindan Xinnaotong soft capsules with 2.4 g·kg-1.Analysis was detected by APCI in multiple reaction monitoring (MRM) mode.Detection of tanshinone ⅡA and salvianolic acid B was using following transitions of m/z 295.0-249.0 for tanshinone ⅡA
m/z 717.1-519.0 for salvianolic acid B and m/z 271.1-122.8 for baicalein.DAS 2.1.1 software was used to calculate pharmacokinetic parameters. Result: Calibration curve ranges were 1-100 and 5-500 μg·L-1 for tanshinone ⅡA and salvianolic acid B
respectively.Mean recovery of them ranged from 72.43% to 93.92%
intra-day and inter-day precisions were in the range of 2.73%-9.87% and accuracies were between 92.65% to 106.26%.Cmax of tanshinone ⅡA and salvianolic acid B were (38.34±17.35)
(32.00±15.43) μg·L-1
Tmax of them were (0.25±0.23)
(0.75±0.18) h. Conclusion: This method developed in the present study using UPLC-MS/MS is highly sensitive
accurate
rapid and suitable for pharmacokinetic study of tanshinone ⅡA and salvianolic acid B from Yindan Xinnaotong soft capsules in rats.
Simultaneous Content Determination of Salvianolic acid A, Salvianolic acid B, Tanshinol, Lithospermum Acid, Tanshinone Ⅱ in Crude Extract of Radix et Rhizoma by HPLC
Analysis of Extraction Technology for Preparations Containing Salviae Miltiorrhizae Radix Et Rhizoma in 2010 Edition of
Optimization of Extraction Process of Qidan Granules by Orthogonal Test
Simultaneous Determination of 7 Components in Salviae Miltiorrhizae Radix et Rhizoma by UPLC-MS/MS
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