LIU Dong-yun, ZHU Guo-xue, WU Chun-wei, et al. Comparison on Pharmacokinetics of Daidzein in Rats After Gavage of Puerariae Lobatae Radix and Compound Naomaitong[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(3): 81-84.
DOI:
LIU Dong-yun, ZHU Guo-xue, WU Chun-wei, et al. Comparison on Pharmacokinetics of Daidzein in Rats After Gavage of Puerariae Lobatae Radix and Compound Naomaitong[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(3): 81-84. DOI: 10.13422/j.cnki.syfjx.2016030081.
Comparison on Pharmacokinetics of Daidzein in Rats After Gavage of Puerariae Lobatae Radix and Compound Naomaitong
Objective: To establish an UPLC-MS method for determining the content of daidzein in rat plasma
and compare pharmacokinetic parameters of daidzein in rats after gavage of compound Naomaitong and Puerariae Lobatae Radix extract for evaluating effects of other ingredients in compound Naomaitong extract on pharmacokinetics of daidzein. Method: Twelve rats were randomly divided into two groups
gastric gavage of compound Naomaitong and Puerariae Lobatae Radix extract according to 1.002 5 mg·kg-1 of daidzein
plasma samples collected at different time
and then using UPLC-MS to determine plasma concentrations of daidzein
pharmacokinetic parameters were calculated by DAS 2.0 software
and its one-way ANOVA with SPSS 16.0 software. Result: There was a good linearity over concentration ranges of 6.175-741.0 μg·L-1
limit of quantitation was 6.175 μg·L-1
the lowest detection limit was 1.235 μg·L-1.Cmax and CL/F of daidzein from compound Naomaitong and Puerariae Lobatae Radix extract were significantly different
the other parameters were not significantly different. Conclusion: This UPLC-MS is sensitive
accurate
specific and applicable to determine in vivo plasma concentration of drug with daidzein as a main ingredient.Other ingredients in compound Naomaitong extract have a great impact on absorption and removal of daidzein
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