Se-enriched  Polysaccharides Improve Nonalcoholic Fatty Liver Disease in Rats by Regulating SCD1 and PPAR

JIA Ya-min; WU Jun-zi; HU Yue-gao; LI Shu-de; SHEN Ping-rui

doi:10.13422/j.cnki.syfjx.2016030102

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Se-enriched Polysaccharides Improve Nonalcoholic Fatty Liver Disease in Rats by Regulating SCD1 and PPAR

更新时间：2024-01-04

- Se-enriched Polysaccharides Improve Nonalcoholic Fatty Liver Disease in Rats by Regulating SCD1 and PPAR
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 22, Issue 3, Pages: 102-106(2016)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2016030102
  CLC： R285.5
- Published：2016
- 稿件说明：
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JIA Ya-min, WU Jun-zi, HU Yue-gao, et al. Se-enriched Polysaccharides Improve Nonalcoholic Fatty Liver Disease in Rats by Regulating SCD1 and PPAR[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(3): 102-106.
DOI：

JIA Ya-min, WU Jun-zi, HU Yue-gao, et al. Se-enriched Polysaccharides Improve Nonalcoholic Fatty Liver Disease in Rats by Regulating SCD1 and PPAR[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(3): 102-106. DOI： 10.13422/j.cnki.syfjx.2016030102.

摘要

目的:探讨富硒灵芝多糖对非酒精性脂肪肝病(NAFLD)大鼠血脂、炎性因子、硬脂酰辅酶A去饱和酶(SCD1)和过氧化物酶体增殖物激活受体α(PPARα)的影响。方法:120只SD大鼠随机分为正常组

高脂饮食组(模型组)

多烯磷脂酰胆碱(50 mg·kg-1)组和富硒灵芝多糖低、中、高剂量(0.3

0.6

1.2 g·kg-1

ig)组

高脂高糖饮食12周复制NAFLD模型

相应治疗4和8周后处死大鼠各半

测定血清中高密度脂蛋白胆固醇(HDL-C)

低密度脂蛋白胆固醇(LDL-C)

白细胞介素-1α(IL-1α)

白细胞介素-1β(IL-1β)

肿瘤坏死因子-α(TNF-α)

肝脏匀浆中SCD1和PPARα含量。结果:处理4周后

与正常组相比

模型组肝脏有明显脂肪空泡

且伴有炎症反应;与模型组相比

富硒灵芝3个治疗组脂肪颗粒明显减少。处理8周后

与正常组相比

模型组HDL-C

LDL-C

IL-1α

IL-1β和TNF-α明显升高

与模型组相比

富硒灵芝多糖3个组的HDL-C

LDL-C

IL-1α

IL-1β和TNF-α明显降低(P<0.05)

治疗8周后进一步降低

与多烯磷脂酰胆碱组无显著差异。处理4周后

与正常组相比

模型组肝脏SCD1和PPARα mRNA和蛋白表达降低

与模型组相比

富硒灵芝多糖3个组肝脏SCD1和PPARα mRNA和蛋白表达明显升高

多烯磷脂酰胆碱处理对肝脏SCD1和PPARα mRNA和蛋白表达改善优于富硒灵芝多糖治疗组。结论:富硒灵芝多糖可改善NAFLD大鼠肝脏SCD1和PPARα表达

效果虽弱于多烯磷脂酰胆碱

但也可较好的调节血脂和炎症。

Abstract

Objective: To investigate the effect of Se-enriched Ganoderma lucidum polysaccharides on blood lipids

inflammatory factors

stearoyl coenzyme1 (SCD1) and peroxisome proliferator-activated receptor α (PPARα) expression in rats with nonalcoholic fatty liver disease (NAFLD). Method: One hundred and twenty SD rats were randomly divided into normal group

high-fat diet group (model group)

polyene phosphatidylcholine(50 mg·kg-1) group

Se-enriched G. lucidum polysaccharides low

medium and high dose groups (0.3

0.6

1.2 g·kg-1

ig). Rats were given high-fat and high-sucrose diet for 12 weeks to establish NAFLD models. After treatment for 4 weeks and 8 weeks

the rats were sacrificed half and half to detect high-density lipoprotein-C (HDL-C)

low-density lipoprotein-C (LDL-C)

interleukin-1α (IL-1α)

interleukin-1β (IL-1β)

tumor necrosis factor-α (TNF-α) in serum

as well as SCD1 and PPARα levels in liver homogenates. Result: Compared with the normal group

fat vacuoles were obvious and associated with inflammation after 4 weeks treatment in model group. Compared with the model group

Se-enriched G. lucidum polysaccharides treatment groups significantly decreased fat particles. Compared with the normal group

HDL-C

LDL-C

IL-1α

IL-1β and TNF-α levels were significantly increased in the model group after 8 weeks of treatment

Compared with the model group

HDL-C

LDL-C

IL-1α

IL-1β and TNF-α levels were significantly decreased in three Se-enriched G. lucidum polysaccharides treatment groups (P<0.05)

and they were further decreased after 8 weeks of treatment

but with no significant difference from the polyene phosphatidylcholine group. Compared with the normal group

SCD1 and PPARα mRNA and protein expressions in liver tissues were significantly decreased in model group after 4 weeks treatment. Compared with the model group

SCD1 and PPARα mRNA and protein expressions in liver tissues were significantly increased in three Se-enriched G. lucidum polysaccharides treatment groups

but the effect of polyene phosphatidylcholine was better than Se-enriched G. lucidum polysaccharides on the improvement of SCD1 and PPARα mRNA and protein expressions. Conclusion: Se-enriched G. lucidum polysaccharides could improve SCD1 and PPARα expressions in liver tissues of NAFLD rats

and could regulate blood lipids

and inflammation although its effect was inferior to polyene phosphatidylcholine.

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