WANG Shan-shan, HU Ping, YU Shao-wen. Optimization of Conditions for Polydatin Being Transformed into Resvertrol by Response Surface Methodology[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(4): 6-10.
DOI:
WANG Shan-shan, HU Ping, YU Shao-wen. Optimization of Conditions for Polydatin Being Transformed into Resvertrol by Response Surface Methodology[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(4): 6-10. DOI: 10.13422/j.cnki.syfjx.2016040006.
Optimization of Conditions for Polydatin Being Transformed into Resvertrol by Response Surface Methodology
Objective: To optimize process of polydatin being transformed into resveratrol by response surface methodology for increacing the content of resveratrol and promoting resource development of Polygoni Cuspidati Rhizoma et Radix. Method: Considering yield of resveratrol as index
based on single factor experiments
response surface methodology was adopted to optimize transformation process by taking the amount of enzyme
temperature
pH and time as factors.UV was employed to determine the content of resveratrol at 305 nm. Result: Optimum technological conditions were as follows:enzyme dosage of 463 μL
temperature at 43 ℃
pH of 5.0
conversion time of 153 min;yield of resveratrol was 16.46 mg · g-1
whose deviation was small by comparing with the theoretical value of 16.52 mg · g-1
which was 3.58 times to before optimization and 1.66 times of the best enzyme hydrolysis process. Conclusion: Response surface analysis is suitable for optimizing transformation process of resveratrol
this process is feasible
which is a beneficial reference for reducing industrial production costs of resveratrol.
Gastrointestinal Absorption Kinetics of Polydatin and Resveratrol from Polygoni Cuspidati Rhizoma et Radix Extract in Rats
Optimization of Extraction Technology of Ganyou Capsules by Multi-index Orthogonal Test
Simultaneous Quantitative Analysis of Polygonin, Resveratrol, Archen and Emodin Monomethyl Ether in Polygoni Cuspidati Rhizoma et Radix by Multi-Components Assay and Single Marker
Simultaneous Determination of Plantamajoside,Polydatin,Specnuezhenide and Resveratrol in Huzhentongfeng Capsule with HPLC
Effect of Polydatin on Growth,Apoptosis, and ROS/p38 MAPK Signaling Pathway of Myeloma Cells
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Related Institution
The Third Hospital of Nanchang
School of Pharmacy,Guangdong Pharmaceutical University
School of Life Sciences and Biopharmaceutics,Guangdong Pharmaceutical University
School of Basic Medical Sciences, Guangzhou University of Chinese Medicine