Optimization of Ethanol Extraction Technology of Xinnaosu Granules by Central Composite Design-response Surface Methodology and Principal Component Analysis

LI Shan-shan; ZHANG Yong-mei; JIANG Zhi-tao; PAN Jin-huo

doi:10.13422/j.cnki.syfjx.2016040015

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Optimization of Ethanol Extraction Technology of Xinnaosu Granules by Central Composite Design-response Surface Methodology and Principal Component Analysis

更新时间：2024-01-04

- Optimization of Ethanol Extraction Technology of Xinnaosu Granules by Central Composite Design-response Surface Methodology and Principal Component Analysis
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 22, Issue 4, Pages: 15-19(2016)
- 作者机构：
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- DOI：10.13422/j.cnki.syfjx.2016040015
  CLC： R283.6
- Published：2016
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LI Shan-shan, ZHANG Yong-mei, JIANG Zhi-tao, et al. Optimization of Ethanol Extraction Technology of Xinnaosu Granules by Central Composite Design-response Surface Methodology and Principal Component Analysis[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(4): 15-19.
DOI：

LI Shan-shan, ZHANG Yong-mei, JIANG Zhi-tao, et al. Optimization of Ethanol Extraction Technology of Xinnaosu Granules by Central Composite Design-response Surface Methodology and Principal Component Analysis[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(4): 15-19. DOI： 10.13422/j.cnki.syfjx.2016040015.

摘要

目的: 优选心脑苏颗粒的醇提工艺条件

为该制剂的成型工艺研究提供参考。方法: 在单因素试验基础上

选择乙醇体积分数、加液量、提取时间为自变量

以抗凝血酶效价、丹参酮ⅡA 和隐丹参酮提取量的总评“归一值”为因变量

通过星点设计-效应面法优选心脑苏颗粒醇提工艺

利用主成分分析法对优选的提取工艺进行分析与验证。采用HPLC测定丹参酮ⅡA和隐丹参酮的含量

流动相甲醇-水(75 :25)

检测波长270 nm。结果: 最佳醇提工艺为加8倍量70%乙醇提取120 min

提取数1次。丹参酮ⅡA 和隐丹参酮的平均提取量分别为0.523

0.225 mg ·g-1

抗凝效价平均值92.599 U ·g-1

总评“归一值”预测值和实测值的偏差2.9%。结论: 优选的提取工艺简单、稳定、可行

为心脑苏颗粒的成型工艺研究奠定基础。

Abstract

Objective: To optimize ethanol extraction technology of Xinnaosu granules and provide a reference for molding technology of this preparation. Method: Based on single factor tests

taking overall desirability of extracting amounts of tanshinone ⅡA and cryptotanshinone

activity of anti-thrombin as index

ethanol concentration

the amount of ethanol and extraction time were selected by central composite design-response surface methodology

extraction technology was analyzed and verified by principal component analysis. HPLC was adopted to determine contents of tanshinone ⅡA and cryptotanshinone with isocratic elution of methanol-water (75 : 25) and detection wavelength at 270 nm. Result: Optimum extraction process conditions was as follows:extracted 120 min with 8 times the amount of 70% ethanol.Extracting amounts of tanshinone ⅡA and cryptotanshinone were 0.523

0.225 mg · g-1

activity of anti-thrombin was 92.599 U · g-1.Overall desirability deviated 2.9% by comparing with the predictive value. Conclusion: This optimized process is simple and reliable with high predictability

which can lay foundation for molding technology of Xinnaosu granules.

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