Objective: To investigate influence of processing on contents of segetalin A

B

E in Vaccariae Semen. Method: HPLC was employed to determine contents of segetalin A

B

E with AcclaimTM 120 C18 column(4.6 mm×250 mm

5 μm)

mobile phase of acetonitrile(A)-water(B) for gradient elution(0-8 min

32%A;8-13 min

32%-60%A;13-20 min

60%-70%A)

flow rate of 1 mL · min-1

detection wavelength at 220 nm

column temperature at 40 ℃

injection volume of 10 μL.Changes of contents of segetalin A

B

E and dissolution rates were analyzed after Vaccariae Semen being processed. Result: After being processed

contents of segetalin A

B

E in processed products of Vaccariae Semen was similar to those of raw herbs

however

their dissolution rates raised 13.2%

5.3% and 17.4%

respectively. Conclusion: Processing has little influence on contents of segetalin A

B

E in Vaccariae Semen

but their dissolution rates increase.