LIU Peng, LIU Yu-hong, LIU Yun-hua, et al. Ion Chromatography Method for Determination of Toxic Ingredients Carboxyatractyloside and Atractyloside in Xanthii Fructus[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(4): 61-64.
DOI:
LIU Peng, LIU Yu-hong, LIU Yun-hua, et al. Ion Chromatography Method for Determination of Toxic Ingredients Carboxyatractyloside and Atractyloside in Xanthii Fructus[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(4): 61-64. DOI: 10.13422/j.cnki.syfjx.2016040061.
Ion Chromatography Method for Determination of Toxic Ingredients Carboxyatractyloside and Atractyloside in Xanthii Fructus
Objective: To establish reversed phase ion pair chromatography method for the determination of two atractyloside toxicants in Xanthii Fructus. This method will shorten the analysis time and improve specificity compared with the former methods. Method: The chromatographic separation was carried out on Agilent Eclipse XDB-C18 column (4.6 mm×250 mm
5.0 μm). The mobile phase was composed of acetonitrile-0.12% tetrabutylammonium hydroxide in 0.15% sodium dihydrogen phosphate solution (pH of phosphoric acid was adjusted to 3.5) (39 : 61). The flow rate was set at 1.0 mL · min-1 and the column temperature was 30 ℃
with detection wavelength of 203 nm. Result: Under the above conditions
two toxicants could be well separated in 20 min (11.8
17.8 min
respectively). The actual sample size had good linear relationship within the range of 0.15-3.7 μg (r=0.999 9) and 0.10-2.5 μg (r=1.000)
respectively. The average recovery rate was 101.4% (RSD 1.7%) and 101.6% (RSD 1.2%)
respectively. Conclusion: This method is quick
accurate
specific and durable
which can be used for safety detection of Xanthii Fructus herbs and their preparations.
Hepatotoxicity Effect of Xanthii Fructus on Immune-sensitive Rat Model Induced by Endotoxin
Effect of Volatile Oil from Xanthii Fructus on Airway Remodeling of Rats with Bronchial Asthma
Pharmacological Effect and Toxicity of Xanthii Fructus
Related Author
Jin ZENG
Zhu-jun YIN
Li LI
Min LIU
Liang-chun YAN
Hua HUA
Jun-ning ZHAO
Xi YAN
Related Institution
Sichuan Academy of Chinese Medicine Sciences, Sichuan Institute for Translational Chinese Medicine, State Key Laboratory of Quality Evaluation of Traditional Chinese Medicine(TCM),Sichuan Geoherbs System Engineering Technology Research Center,Sichuan Provincial Key Laboratory of Quality Evaluation of TCM and Innovative Chinese Medicine Research