Objective: To establish a method of quantitative analysis of multi-components by single marker (QAMS) for the determination of L-hydroxyproline

glycine

alanine and proline in Asini Corii Colla. Method: With L-hydroxyproline as the internal reference substance

the relative correction factors (RCF) of glycine

proline and alanine were determined by HPLC and were used to calculate corresponding contents.At the same time

the contents of the four amino acids were determined by external standard method

and the calculated values and estimated values were compared to ensure the accuracy and feasibility of QAMS. Result: The experiments showed good linear relationship at the determination ranges: L-hydroxyprolinem 0.017-0.26 μg (r=0.999 8)

glycine 0.033-0.50 μg (r=0.999 7)

alanine 0.015-0.23 μg (r=0.999 5) and proline 0.025-0.37 μg (r=0.999 8). The average recoveries of four componts were from 96.8% to 98.4% and the relative standard deviations were all less than 3.0%. The RRT of glycine

alanine and proline were 1.386

1.940 and 2.129

and the RCF were 0.642

0.975 and 0.951

respectively. Calculated values of QAMS and estimated values of external standard method did not make significant difference. It showed that the RCF was authentic. Conclusion: QAMS has the applicability and feasibility for the determination of the four amino acids ingredients in Asini Corii Colla.