ZHANG Liang-zhi, CHANG Xue-hui, NING Ya-hong. Effect of Guiling Pa'an Pill on Expression of Nestin, Nestin mRNA and DAT in Substantia Nigra Cells of Rat Models with Parkinson's Disease[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(4): 115-118.
DOI:
ZHANG Liang-zhi, CHANG Xue-hui, NING Ya-hong. Effect of Guiling Pa'an Pill on Expression of Nestin, Nestin mRNA and DAT in Substantia Nigra Cells of Rat Models with Parkinson's Disease[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(4): 115-118. DOI: 10.13422/j.cnki.syfjx.2016040115.
Effect of Guiling Pa'an Pill on Expression of Nestin, Nestin mRNA and DAT in Substantia Nigra Cells of Rat Models with Parkinson's Disease
Objective: To investigate the effect of Guiling Pa'an pill on the expression of Nestin
Nestin mRNA and dopamine transporter (DAT) in substantia nigra cells of rats with Parkinson's disease (PD). Method: SPF grade rats were stereotaxically injected with 6-hydrox-ydopamine (6-OHDA) and apomorphinum (APO) to induce PD rat models. Rats were randomly divided into model group
madopar group
Guiling Pa'an pill high dose group
middle dose group and low dose group
and at the same time
the sham operation group was also established. Rats in model group and sham operation group daily received the same volume of normal saline. Rats in madopar group received 10 mg · kg-1 · d-1 suspension by ig. Rats in Guiling Pa'an pill high dose group
middle dose group and low dose group received 4
2
1 g · kg-1 · d-1 suspension respectively by ig
all with a course of 30 days. The Nestin and DAT protein levels of the substantia nigra tissue were detected by immunohistochemistry for various groups;Nestin mRNA level in substantia nigra tissues was detected by hybridization in situ method for various groups. Result: Nestin and DAT protein expression and Nestin mRNA expression in substantia nigra tissues of the model group were significantly lower than those in sham operation group (P<0.01). Compared with model group
the Nestin protein
Nestin mRNA and DAT protein expressions in substantia nigra tissues of various treatment groups were significantly increased (P<0.01). Conclusion: Action mechanism of Guiling Pa'an pill for the treatment of Parkinson's disease might be associated with increasing Nestin
DAT expressions
which would promote the repair and regeneration of nerves and increase the content of dopamine.
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