Objective: To study the regulatory mechanism of Wenjing Xiaocuo granules on sex hormone in an animal experiment. Method: Totally 40 SD rats were randomly divided into blank group

tanshinone group (0.4 g · kg-1 · d-1)

Wenjing Xiaocuo granules of low

medium and high dose groups (0.4 g · kg-1 · d-1)

with 8 rats in each group. Wenjing Xiaocuo granules low

medium and high dose groups and tanshinone group were gavaged corresponding Wenjing Xiaocuo granules solution and tanshinone solution

blank group was gavaged distilled water for 7 days. Seven days later

efforts were made to take blood from the abdominal aorta

and cut off the uterus and weigh it

then calculate the coefficient of uterus and observe uterine pathology. IHC was used to detect the changes of estrogen receptor α (ERα) and estrogen receptor β (ERβ)

ELISA was used to detect the content of estradiol (E2) and testosterone (T) in the rats. Result: In all of treatment groups

uterus coefficient increased

particularly the Wenjing Xiaocuo granules medium and high dose group (P<0.01). In all of treatment groups

uterine tissue hyperplasia was obvious

epithelial monolayer became stratified

the epithelial thickness also increased significantly. With respect to E2 level

compared with the blank group

all other groups were significantly higher (P<0.05

P<0.01)

particularly Wenjing Xiaocuo granules high dose group. The content of T in high dose group was the lowest (P<0.01). Compared with blank group

the expressions of ERα and ERβ of all other groups were significantly increased (P<0.01)

and the expressions of all Wenjing Xiaocuo granules groups increased with the rise in dose. Conclusion: Wenjing Xiaocuo granules can promote the secretion of estrogen

suppress the secretion of androgen

and increase the expression of estrogen receptor

with the effect enhancing with the increasing dose.