Effect of Banxia Xiexin Tang on Cell Cycle, Apoptosis and STAT3 Protein Expression of Esophageal Carcinoma Eca9706 Cells

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Effect of Banxia Xiexin Tang on Cell Cycle, Apoptosis and STAT3 Protein Expression of Esophageal Carcinoma Eca9706 Cells

更新时间：2024-01-04

- Effect of Banxia Xiexin Tang on Cell Cycle, Apoptosis and STAT3 Protein Expression of Esophageal Carcinoma Eca9706 Cells
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 22, Issue 4, Pages: 142-145(2016)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2016040142
  CLC： R285
- Published：2016
- 稿件说明：
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CUI Shan-shan, SHAO Lei, GAO Xiao-ling, et al. Effect of Banxia Xiexin Tang on Cell Cycle, Apoptosis and STAT3 Protein Expression of Esophageal Carcinoma Eca9706 Cells[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(4): 142-145.
DOI：

CUI Shan-shan, SHAO Lei, GAO Xiao-ling, et al. Effect of Banxia Xiexin Tang on Cell Cycle, Apoptosis and STAT3 Protein Expression of Esophageal Carcinoma Eca9706 Cells[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(4): 142-145. DOI： 10.13422/j.cnki.syfjx.2016040142.

摘要

目的: 观察半夏泻心汤对食管癌Eca9706凋亡机制的影响。方法: 体外培养食管癌Eca9706细胞

采用流式细胞术观察半夏泻心汤(4

2

1 g ·L-1)对食管癌Eca9706细胞周期及凋亡的影响

采用免疫印迹法(Western blot)检测半夏泻心汤对Eca9706细胞信号转导和转录激活因子3(signal transducer and activator of transcription 3

STAT3)蛋白的表达。结果: 半夏泻心汤各剂量作用于食管癌Eca9706细胞24 h后

食管癌Eca9706细胞早期凋亡明显增加(P< 0.05)

食管癌Eca9706细胞分裂被明显阻止于休眠细胞/DNA合成前期(G0/G1);半夏泻心汤各剂量作用于食管癌Eca9706 24 h后

可使细胞STAT3蛋白表达明显降低(P< 0.05)

以高浓度半夏泻心汤效果最佳。结论: 半夏泻心汤可能存在影响食管癌Eca9706细胞周期(细胞分裂间期G1

S

G2/M期之间)的调控点;半夏泻心汤促进肿瘤细胞凋亡可能与干预STAT3转录因子表达有关。

Abstract

Objective: To observe the apoptosis mechanism of Banxia Xiexin Tang on esophageal carcinoma Eca9706 cells. Method: Flow cytometry technique was used to detect the effect of Banxia Xiexin Tang on cell cycle and apoptosis of esophageal carcinoma Eca9706 cells;Western blot was used to detect the effect of Banxia Xiexin Tang on signal transducer and activator of transcription 3 (STAT3) protein expression of Eca9706 cells. Result: After 24 h of treatment by Banxia Xiexin Tang

the early apoptosis of cells was significantly increased

and cell division was significantly blocked in G0/G1 phase

STAT3 protein expression was reduced after 24 h of treatment by Banxia Xiexin Tang

and the high concentration Tang exhibited the best effect. Conclusion: Banxia Xiexin Tang may have the regulation points between G1

S and G2/M phase to affect cell cycle

and its mechanism of promoting apoptosis of tumor cells may be associated with interfering the expression of STAT3 transcription factors.

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