CHEN Xiu-hong, REN Jin-hong, WEI Yan-ming, et al. Optimization of Extraction Technology of An Immune Active Protein from Astragali Radix[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(5): 13-17.
DOI:
CHEN Xiu-hong, REN Jin-hong, WEI Yan-ming, et al. Optimization of Extraction Technology of An Immune Active Protein from Astragali Radix[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(5): 13-17. DOI: 10.13422/j.cnki.syfjx.2016050013.
Optimization of Extraction Technology of An Immune Active Protein from Astragali Radix
加16倍量pH 8.0的三羟甲基氨基甲烷盐酸盐(Tris-HCl)于40 ℃常压水浴提取60 min
每10 min搅拌1次.AmPR10-16提取率0.063 g ·g-1. 结论: 优化的提取工艺能正确反映AmPR10-16提取率的最高相对量
为AmPR10-16的进一步研究提供了稳定、合理、可行的提取工艺.
Abstract
Objective: To optimize extraction technology of an immune active protein (AmPR10-16) with a molecular weight of 16.0 kDa from Astragali Radix. Method: Extraction temperature was investigated by circular dichroism of water-soluble protein involving in AmPR10-16 with secondary structure from Astragali Radix.Extraction technology was optimized by single factor test and orthogonal test with gray value of AmPR10-16 as index which was determined by gel graphical analysis software. In this study
effects of temperature
solid-liquid ratio
time
solvent
granularity and times on gray value were investigated
for which determination of water-soluble protein was determined as an evidence by BCA method. Result: Optimum extraction process of AmPR10-16 was as follows:with Tris-HCl with pH 8.0 as solvent
solid-liquid ratio of 1 : 16
extraction time of 60 min with temperature at 40 ℃
medicinal powder over the 4th sieve
stirring once every 10 min.Yield of AmPR10-16 was 0.063 g · g-1. Conclusion: This optimal extraction technology can accurately reflect relative amounts of AmPR10-16 maximum extraction rate
it provides a stable
reasonable and feasible extraction process for furter study of AmPR10-16.