Objective: To establish the fingerprints of Shuanglianfang based on full-time multi-wavelength fusion method

and provide basis for the quality control of Shuanglianfang. Method: High performance liquid chromatography (HPLC) method was used on Welch Ultimate LP-C18 column (4.6 mm×250 mm

5 μm)

at the mobile phase of 0.1% methanoic acid solution (A) and ethanol (B) for gradient elution (0-20 min

25%-60%A; 20-40 min

60%-65%A;40-70 min

65%-45%A). The flow rate was maintained at 1.0 mL · min-1and the temperature was maintained at 30 ℃. Detector wavelengths were 254

300

320

365 nm. Result: With the chromatographic peak of luteolin as the reference peak

18 chromatographic peaks in total were identified

and the full-time multi-wavelength fusion fingerprints of Shuanglianfang were established. Based on the HPLC fingerprints

relative quantification of other components in Shuanglianfang was conducted

and the content of other components in Shuanglianfang shall not be less than 0.39

0.24

0.46

0.45

0.73

0.28

0.16

0.41

0.51

2.37

0.77

0.22

0.60

0.36

0.62

0.80

0.52

and 0.96 relative to luteolin. Conclusion: This method is simple

accurate

scientific

and suitable for the quality evaluation of Shuanglianfang.