Objective: To determine the content of volatile index component limonene in Qizhi Weitong granules

and improve the quality control standard of volatile components in Qizhi Weitong granules compound. Method: In this study

high performance liquid chromatography (HPLC) was used to investigate the optimum pretreatment process of limonene in sugarless Qizhi Weitong granules

and a methodological study was done for this method.At the same time

content of limonene in 10 batches of Qizhi Weitong granules was detected and their content limits were determined

and to establish a rapid identification by infrared spectroscopy easy way limonene content. Result: The obtained optimum pretreatment method for limonene was as follows: take sugarless Qizhi Weitong granules 2.5 g

add methanol 50 mL

and with ultrasonic treatment (power 250 W

frequency 40 kHz) for 30 min. Methodological study identified a linear range of limonene between 0.17-1.68 μg (r=0.999 8)

and the average recovery rate was 99.77%

and the content limit of limonene in Qizhi Weitong granules was determined as not less than 1.00 mg. Gray correlation analysis were obtained 3 and limonene content related peaks. Conclusion: The method is simple

scientific and reasonable

and can be used as a method for the determination of limonene content in Qizhi Weitong granules. The present study has great significance for quality evaluation of Qizhi Weitong granules

and provides great supplementary for the full and comprehensive evaluation of traditional Chinese medicine prescriptions. The study combined data analysis of gray theory

construction of the coarse particle inspection Qizhiweitong limonene content of infrared spectroscopy

for the rapid identification of stomach Qi stagnation particles limonene provides an effective

simple and intuitive way

for the full

comprehensive evaluation of traditional Chinese medicine prescription preparations Qizhiweitong the quality of the particles provide a strong complement.