Objective: To observe the pharmacodynamic effect of Zijin Huadu suppository on treatment of cervicitis induced by virus

bacteria and phenol in vivo and in vitro

and provide experimental basis for evaluating its therapeutic effects for cervicitis. Method: The 70 SD rats were randomly divided into normal group

model group

Funing suppository group (0.15 g · kg-1 · d-1)

policresulen suppository group (8.4 mg · kg-1 · d-1)

Zijin Huadu suppository high dose group

middle dose group and low dose group (1.2

0.6

0.3 g · kg-1 · d-1)

n=10 in each group. Needle scraping was done for three times in all the other groups except the normal group to induce surface damage of uterus

and infusion was done once every 2 days for 3 times in total to induce infection models. After successful modeling

vagina administering was done once a day for continuous 12 days. HE staining pathological dyeing was used to observe the cervicitis lesions of rats;ELISA assay was used to detect the expression of virus in cervical tissues;in vitro test was used to observe its inhibitory effect on herpes simplex virus-2 (HSV-2); RT-PCR and CPE method were used to observe the inhibitory effect of Zijin Huadu suppository on virus replication and its bactriostasis activity was observed by turbidimetry. Result: The results demonstrated that in HPV16-infected cervicitis rat models

Zijin Huadu suppositories at doses of 1.2

0.6

0.3 g · kg-1 · d-1had significant inhibitory effect on expression of HPV16 in cervical tissues

and the doses of 1.2

0.6 g · kg-1 · d-1 had significant inhibitory effect on pathological changes of cervical tissues. In cervicitis rat models with mixed infection of Escherichia coli

Neisseria gonorrhoeae and Staphylococcus aureus

Zijin Huadu suppositories at doses of 1.2

0.6 g · kg-1 · d-1 had significant inhibitory effect on pathological changes of cervical tissues. In cervicitis rat models induced by chemical substances

Zijin Huadu suppositories at dose of 1.2 g · kg-1 · d-1 had significant inhibitory effect on vagina and cervix lesions in rats. In vitro

Zijin Huadu suppository showed obvious inhibitory effect on HSV-2

HPV16

S. aureus

S. albus

S. epidermidis

group B Streptococcus

E. coli

Pseudomonas aeruginosa

Proteusbacillus vulgaris

Neisseria gonorrhoeae and Candida albicans. Conclusion: Zijin Huadu suppository has obvious inhibitory effect in vitro on viruses and bacteria

and has obvious improvement for all the phenol-induced cervicitis.