Effect of Resveratrol on Expression of Bad, p-Bad and Caspase-3 Protein in MGC803 Cells

JING Xiao-ping; CHENG Wei-wei; HE Li

doi:10.13422/j.cnki.syfjx.2016050147

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Effect of Resveratrol on Expression of Bad, p-Bad and Caspase-3 Protein in MGC803 Cells

更新时间：2024-01-04

- Effect of Resveratrol on Expression of Bad, p-Bad and Caspase-3 Protein in MGC803 Cells
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 22, Issue 5, Pages: 147-150(2016)
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- DOI：10.13422/j.cnki.syfjx.2016050147
  CLC： R285
- Published：2016
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JING Xiao-ping, CHENG Wei-wei, HE Li. Effect of Resveratrol on Expression of Bad, p-Bad and Caspase-3 Protein in MGC803 Cells[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(5): 147-150.
DOI：

JING Xiao-ping, CHENG Wei-wei, HE Li. Effect of Resveratrol on Expression of Bad, p-Bad and Caspase-3 Protein in MGC803 Cells[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(5): 147-150. DOI： 10.13422/j.cnki.syfjx.2016050147.

摘要

目的: 探讨白藜芦醇(resveratrol

Res)对人胃癌细胞MGC803细胞凋亡因子及其磷酸化的影响

研究白藜芦醇抗胃癌的作用机制. 方法: 采用0

100

200 μmol ·L-1 Res处理MGC803细胞后

采用台盼蓝法测定MGC803细胞生长抑制率;流式细胞术(FCM)检测细胞凋亡率;免疫组化法检测促凋亡蛋白(Bad)

含半胱氨酸的天冬氨酸蛋白水解酶-3(Caspase-3)蛋白表达;蛋白质印迹法(Western blot)检测Bad

磷酸化Bcl-x1/Bcl-2相关死亡启动子(p-Bad)

Caspase-3蛋白的表达. 结论: 随着浓度增加Res抑制胃癌MGC803细胞生长作用明显增强(P<0.01);Res(100 μmol ·L-1)能明显下调MGC803细胞中Bad

p-Bad蛋白表达

同时可上调Caspase-3蛋白表达. 结论: Res可以诱导MGC803细胞凋亡

其凋亡与浓度、时间有一定的依赖性;Res诱导MGC803细胞凋亡可能与其下调Bad

p-Bad蛋白和上调Caspase-3蛋白表达有关.

Abstract

Objective: To observe the effects of resveratrol (Res) on MGC803 cells apoptosis and study its mechanism of inhibiting gastric cancer. Method: After MGC803 cells were treated with 0

100

200 μmol · L-1 Res

inhibition rate of MGC803 cells growth was detected by trypan blue assay. Apoptosis rate was detected by flow cytometry (FCM). Expressions of Bad and Caspase-3 protein in MGC803 cells were measured by immunohistochemistry method. Expressions of Bad

p-Bad

Caspase-3 protein were detected by Western blot. Result: Res inhibited the growth of MGC803 cells in time-and concentration-dependent manner (P<0.01). Res (100 μmol · L-1) can significantly down-regulate the protein expressions of Bad

p-Bad and up-regulate the protein expression of Caspase-3 in MGC803 cells. Conclusion: Res can induce apoptosis of MGC803 cells in time-and concentration-dependent manner

and the mechanism may be associated with down-regulating protein expressions of Bad

p-Bad and up-regulating protein expressions of Caspase-3.

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