TIAN Li-li, SHENG Dong-lai, ZHU Guo-fu. Inhibiting Effect and Mechanism of Oridonin on Angiogenesis Activity[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(5): 166-171.
DOI:
TIAN Li-li, SHENG Dong-lai, ZHU Guo-fu. Inhibiting Effect and Mechanism of Oridonin on Angiogenesis Activity[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(5): 166-171. DOI: 10.13422/j.cnki.syfjx.2016050166.
Inhibiting Effect and Mechanism of Oridonin on Angiogenesis Activity
Objective: To study the effect and mechanism of oridonin on inhibiting angiogenesis. Method: In vitro and in vivo models were used in this paper to study the anti-angiogenic effects of oridonin. Through in vitro models
methyl thiazolyl tetrazolium (MTT) assay was ysedti detect the cells vitality and enzyme-linked immunosorbent assay (ELISA) was used to determine vascular endothelial growth factors level (VEGF) in cardiac microvascular endothelial cells (CMEC). Through in vivo models
transgenic zebrafish models (Fli1-GFP) were used to observe the effect of oridonin on angiogenesis of embryonic period and regeneration after vascular injury in adult zebrafish. Expressions of main related genes VEGFA
VEGFR2 and VEGR3 in VEGF pathways were detected with relative fluorescence quantitative PCR method. Result: Oridonin inhibited angiogenesis and endothelial cells in vitro
with IC50 of 8.04 mg · L-1
and significantly reduced VEGF expression in the cell serum. What's more
oridonin inhibited angiogenesis in zebrafish embryos vascular assay and inhibited regeneration after vascular injury in adult zebrafish. Our study elucidated the mechanism of the anti-angiogenic activity of oridonin at least in part
which may be related to down-regulating the expression of VEGFA
VEGFR2 and VEGR3 genes. Conclusion: Oridonin can effectively inhibit angiogenesis
which will provide greater contribution for anti-tumor angiogenesis therapy.
Progress of Anti-osteoporosis Research of Traditional Chinese Medicine Based on Zebrafish Model
Evaluation of Anti-osteoporosis Activity and Hepatotoxicity of Xianling Gubao Based on Zebrafish Model
Hepatotoxicity and Anti-osteoporotic Mechanism of Evodiamine
Evaluation of Safety of Compound Kushen Injection and Efficacy in Treatment of Inflammatory Bowel Disease Based on Zebrafish Model
Application of Zebrafish in Quality Evaluation of Traditional Chinese Medicine
Related Author
WANG Henghong
FAN Xinyu
GAO Yihan
LUO Zhilue
DUAN Peng
ZHOU Yunfeng
LI Qiuman
QIAN Yue
Related Institution
Key Laboratory of Zebrafish Modeling and Drug Screening for Human Diseases of Xiangyang City, Xiangyang No.1 People's Hospital, Hubei University of Medicine, Xiangyang
Xiangyang No.1 People′s Hospital, Hubei Universi ty of Medicine, Xiangyang
Beijing Key Laboratory of Quality Evaluation of Traditional Chinese Medicine, School of Chinese Materia Medica, Beijing University of Chinese Medicine