Simultaneous Determination of Eleven Major Alkaloids in Corydalis Rhizoma by RP-HPLC

YANG Min; LI Chun; FENG Wei-hong; ZHANG Yong-xin; LIN Li-mei; CHEN An-jia; YANG Li-xin

doi:10.13422/j.cnki.syfjx.2016060022

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Simultaneous Determination of Eleven Major Alkaloids in Corydalis Rhizoma by RP-HPLC

更新时间：2024-01-04

- Simultaneous Determination of Eleven Major Alkaloids in Corydalis Rhizoma by RP-HPLC
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 22, Issue 6, Pages: 22-25(2016)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2016060022
  CLC： O657.72;R284.1
- Published：2016
- 稿件说明：
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YANG Min, LI Chun, FENG Wei-hong, et al. Simultaneous Determination of Eleven Major Alkaloids in Corydalis Rhizoma by RP-HPLC[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(6): 22-25.
DOI：

YANG Min, LI Chun, FENG Wei-hong, et al. Simultaneous Determination of Eleven Major Alkaloids in Corydalis Rhizoma by RP-HPLC[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(6): 22-25. DOI： 10.13422/j.cnki.syfjx.2016060022.

摘要

目的: 建立RP-HPLC同步测定延胡索中11种生物碱成分的方法

为该药材的质量控制提供参考。方法: Kromasil Eternity C18色谱柱(4.6 mm×250 mm

5 μm)

流动相乙腈(A)-0.1 mol·L-1乙酸铵溶液(每1 L溶液中加冰乙酸0.5 mL)(B)梯度洗脱(0~50 min

20%~55%A;50~55 min

55%~65%A;55~60 min

65%A)

流速1.0 mL·min-1

柱温25 ℃

检测波长280 nm

进样量10 μL。结果: 11种待测成分的线性关系良好

相关系数均>0.999;精密度试验RSD 0.1%~2.0%

稳定性试验RSD 0.2%~2.1%

重复性试验RSD 1.7%~4.1%

平均加样回收率98.2%~100.8%(RSD 1.6%~3.5%)

均符合中药质量分析要求。结论: 建立的RP-HPLC灵敏、简便、可靠

可用于延胡索药材中多种生物碱成分的同步测定。

Abstract

Objective: An efficient method using RP-HPLC was developed for simultaneous determination of eleven major active alkaloids in Corydalis Rhizoma. Method: Chromatographic separation was achieved on a Kromasil Eternity C18 column (4.6 mm×250 mm

5 μm) with a gradient mobile phase of acetonitrile-0.1 mol·L-1 ammonium acetate solution (added 0.5 mL of glacial acetic acid per 1 L) at a flow rate of 1.0 mL·min-1

detection wavelength was set at 280 nm and column temperature was at 25 ℃. Result: Eleven major alkaloids showed a good linearity with r>0.999 and good precision (RSD of 0.1%-2.0%)

stability (RSD of 0.2%-2.1%)

repeatability (RSD of 1.7%-4.1%);average recovery was 98.2%-100.8% with RSD of 1.6%-3.5%. Conclusion: This established method is sensitive

convenient and reliable for determining eleven major components in Corydalis Rhizoma.

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