Content Determination on Five Active Ingredients in Five Species Geranii Herba of Liaoning Provience

GU Jing-yi; YIN Hai-bo; ZHAO Rong; JIA Xiao-qing; SHAO Fei

doi:10.13422/j.cnki.syfjx.2016060026

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Content Determination on Five Active Ingredients in Five Species Geranii Herba of Liaoning Provience

更新时间：2024-01-04

- Content Determination on Five Active Ingredients in Five Species Geranii Herba of Liaoning Provience
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 22, Issue 6, Pages: 26-29(2016)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2016060026
  CLC： O657.72;R284.1
- Published：2016
- 稿件说明：
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GU Jing-yi, YIN Hai-bo, ZHAO Rong, et al. Content Determination on Five Active Ingredients in Five Species Geranii Herba of Liaoning Provience[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(6): 26-29.
DOI：

GU Jing-yi, YIN Hai-bo, ZHAO Rong, et al. Content Determination on Five Active Ingredients in Five Species Geranii Herba of Liaoning Provience[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(6): 26-29. DOI： 10.13422/j.cnki.syfjx.2016060026.

摘要

目的: 建立HPLC同时测定老鹳草属5种老鹳草中原儿茶酸、没食子酸、柯里拉京、鞣花酸和金丝桃苷含量的方法。方法: 采用AgilentTC-C18色谱柱(4.6 mm×250 mm

5 μm)

流动相0.1%磷酸-乙腈梯度洗脱

流速1.0 mL·min-1

检测波长280 nm

柱温30 ℃。结果: 原儿茶酸、没食子酸、柯里拉京、鞣花酸和金丝桃苷5种成分的线性范围分别为0.500~5.000 μg(r=0.999 5)

0.075~0.750 μg(r=0.999 7)

1.790~17.900 μg(r=0.999 9)

0.580~5.800 μg(r=0.999 6)

0.065~0.650 μg(r=0.999 6)

平均回收率在94.05%~102.58%

RSD在1.6%~2.9%。结论: 该方法简单、准确

具有良好的重复性和稳定性

可为老鹳草药材的质量评价提供一定的参考依据。

Abstract

Objective: To establish an HPLC method to determine the content of protocatechuic acid

gallic acid

corilagin

ellagic acid and hyperoside in Geranii Herba. Method: HPLC column Agilent TC-C18(4.6 mm×250 mm

5 μm) was eluted with the

mobile phase of 0.1% phosphonic acid-acctonitril in a gradient mode

the flow rate was 1.0 mL·min-1

the detection wave-length was 280 nm

and the temperature was 30 ℃. Result: The linear ranges of the 5 ingredients in HPLC were 0.500-5.000 μg (r=0.999 5)

0.075-0.750 μg (r=0.999 7)

1.790-17.900 μg (r=0.999 9)

0.580-5.800 μg (r=0.999 6)

0.065-0.650 μg (r=0.999 6)

the ranges of average recoveries and RSD were 94.05%-102.58% and 1.6%-2.9%. Conclusion: The method is simple and accurate with a high repeatability

which could provide reference for the quality evaluation of Geranii Herba.

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Related Institution

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School of Life Sciences，Beijing University of Chinese Medicine

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Affiliated Hospital of Nanjing University of Chinese Medicine/Jiangsu Province Hospital of Chinese Medicine

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