Content Determination of Rutin and Quercetin in  from Different Producing Areas by HPLC

PENG Liang; LI Yi-guang; CHEN Jie; RAO Yi; JI Qiao-yu; WEI Hui-zhen

doi:10.13422/j.cnki.syfjx.2016060045

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Content Determination of Rutin and Quercetin in from Different Producing Areas by HPLC

更新时间：2024-01-04

- Content Determination of Rutin and Quercetin in from Different Producing Areas by HPLC
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 22, Issue 6, Pages: 45-47(2016)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2016060045
  CLC： O657.72;R284.1
- Published：2016
- 稿件说明：
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PENG Liang, LI Yi-guang, CHEN Jie, et al. Content Determination of Rutin and Quercetin in from Different Producing Areas by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(6): 45-47.
DOI：

PENG Liang, LI Yi-guang, CHEN Jie, et al. Content Determination of Rutin and Quercetin in from Different Producing Areas by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(6): 45-47. DOI： 10.13422/j.cnki.syfjx.2016060045.

摘要

目的: 建立高效液相色谱同时测定不同产地、不同品种绞股蓝药材中芦丁和槲皮素含量的方法。方法: ZORBAX SB-C18色谱柱(4.6 mm×150 mm

5 μm)

流动相乙腈(A)-0.2%磷酸溶液(B)梯度洗脱

流速1.0 mL·min-1

柱温30 ℃

检测波长360 nm

进样量10 μL。结果: 芦丁在2.535~10.14 μg线性良好(R2=0.999 2)

平均回收率为98.72%

RSD 1.2%;槲皮素在0.018 1~0.144 8 μg线性良好(R2=0.999 3)

平均回收率98.40%

RSD 1.7%。结论: 该方法简便可行、重复性好

适用于绞股蓝药材2种有效成分的同时测定

可为绞股蓝药材的质量控制提供参考依据。

Abstract

Objective: To establish a determination method for different varieties of rutinand quercetin in Gynostemma pentaphyllum from different producing areas. Method: HPLC was performed on ZORBAX SB-C18column (4.6 mm×150 mm

5 μm)

eluting in a gradient mode with the mobile phase of acetonitrile (A ) -0.2%H3PO4 as (B) at a flow rate of 1.0 mL·min-1. The column temperature was 30 ℃. The detection wavelength was 360 nm. The injection volume was 10 μL. Result: The linearity of rutin was good within the range of 2.535-10.14 μg (R2=0.999 2)

and the average recovery was 98.72%(RSD 1.2%). The linearity of quercetin was good within the range of 0.018 1-0.144 8 μg (R2=0.999 3)

and the average recovery of 98.40%(RSD 1.7%). Conclusion: The established method is simple

feasible and highly reproducible

and can be used for determining rutin and quercetin in G. pentaphyllum and providing reference for quality control of G. pentaphyllum.

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Related Institution

Department of Pharmacy,Leshan Vocational Technical College

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