Studies on Volatile Constituents of 11 Families of  Flowers

LYU Su-hua; XU Meng; ZHANG Xin-feng; LIU Jing-jing; SI Jin-ping

doi:10.13422/j.cnki.syfjx.2016060052

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Studies on Volatile Constituents of 11 Families of Flowers

更新时间：2024-01-04

- Studies on Volatile Constituents of 11 Families of Flowers
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 22, Issue 6, Pages: 52-57(2016)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2016060052
  CLC： O657.63;R284.1
- Published：2016
- 稿件说明：
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LYU Su-hua, XU Meng, ZHANG Xin-feng, et al. Studies on Volatile Constituents of 11 Families of Flowers[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(6): 52-57.
DOI：

LYU Su-hua, XU Meng, ZHANG Xin-feng, et al. Studies on Volatile Constituents of 11 Families of Flowers[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(6): 52-57. DOI： 10.13422/j.cnki.syfjx.2016060052.

摘要

目的: 为了对铁皮石斛主栽品种森山1号和11个杂交家系鲜花进行挥发性成分分析

并鉴定香气成分组成

比较不同家系铁皮石斛花的挥发性成分差异

同时也能够为进一步完善铁皮石斛新品种选育提供技术支持。方法: 采用顶空固相微萃取(solid phase micro-extraction

HS-SPME)技术和气相色谱-质谱联用(GC-MS)法对铁皮石斛花中挥发性成分进行提取分析

将检测到的不同家系铁皮石斛花成分组成及含量通过SPSS 18.0软件进行聚类分析

分析亲缘关系的远近。结果: 铁皮石斛花挥发性成分主要以萜类、脂肪族、芳香族化合物为主

各类型成分在不同品种及家系中的分布存在着一定差异。聚类分析结果基于亲缘关系较近的家系在性状及挥发性成分组成上都较为接近

11个家系铁皮石斛按亲缘关系被分为3类。结论: 固相微萃取技术在保证充分检测物质含量的基础上

将挥发性物质的流失降低到最小

简便精准。同时利用铁皮石斛鲜花挥发性成分组成和亲缘关系聚类可为铁皮石斛杂交选育及品系评价提供理论参考。

Abstract

Objective: In order to analyze the volatile constituents in cultivars Senshan 1 and the other 11 Dendrobium officinale fresh flowers

and identify of aroma chemical composition. The volatile components compared the in different D.officinale flowers

should be used as an indicator to provide a theoretical reference for D.officinale strain evaluation. Method: Extract and analyze the volatile components through head space solid phase micro-extraction (HS-SPME) and GC-MS

and then through SPSS 18.0 software to judge distance kinship using the volatile components in each D.officinale flowers. Result: The main compounds include terpenes

esters

aliphatic

aromatic compounds

various types volatile components appeared differences among 11 families of D.officinale flowers. The cluster analysis results based on close kinship families are relatively close in properties and composition of the volatile components

then the 11 families Dendrobium should divided into three categories according to kinship clustering. Conclusion: The advantage of solid phase micro extraction technology ensure the full content material content and reduce the loss of volatile substances. And at the same time take advantage of the volatile chemical composition and phylogenetic relationships can also provide theoretical reference for D.officinale hybrid breeding and strain evaluation.

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