TLC Identification and Determination of Quercetin in

LIU Liang; FENG Hua; LIU Ying-bo; PAN Nian-song; ZHOU De-quan

doi:10.13422/j.cnki.syfjx.2016060058

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TLC Identification and Determination of Quercetin in

更新时间：2024-01-04

- TLC Identification and Determination of Quercetin in
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 22, Issue 6, Pages: 58-60(2016)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2016060058
  CLC： R29
- Published：2016
- 稿件说明：
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LIU Liang, FENG Hua, LIU Ying-bo, et al. TLC Identification and Determination of Quercetin in [J]. Chinese journal of experimental traditional medical formulae, 2016, 22(6): 58-60.
DOI：

LIU Liang, FENG Hua, LIU Ying-bo, et al. TLC Identification and Determination of Quercetin in [J]. Chinese journal of experimental traditional medical formulae, 2016, 22(6): 58-60. DOI： 10.13422/j.cnki.syfjx.2016060058.

摘要

目的: 建立苗族药夜关门的薄层鉴别与含量测定方法。方法: 采用薄层色谱法

以甲苯-甲酸乙酯-甲酸 (10:8:1) 为展开剂

建立了其定性鉴别方法;采用高效液相色谱法

以槲皮素为对照

Hypersil ODS2 C18色谱柱(4.6 mm×200 mm

5 μm)

流动相乙腈-0.20%磷酸溶液(20 :80)

二极管矩阵检测器

建立了其含量测定方法。结果: 建立的TLC能很好分离各成分

HPLC能够准确测定苗药夜关门中槲皮素的含量。结论: 该文所建立的TLC

HPLC方法可用于苗药夜关门质量检测。

Abstract

Objective: To establish methods of thin layer chromatography (TLC) identification and content determination of Lespedeza cuneata. Method: Quality differentiation method was developed using TLC and with toluene-ethyl formate-formic acid (10 : 8 : 1) as the developing solvent. Using high performance liquid chromatography (HPLC)

content determination of quercetin was carried on Hypersil ODS2 C18(4.6 mm×200 mm

5 μm) column eluted with a mixture of acetonitrile-0.2% phosphate(20 : 80) as the mobile phase

and diodematrix detector was used as the detector. Result: The established TLC method could effectively isolate each component

and the established HPLC method could accurately determine the content of quercetin in L cuneata. Conclusion: The established methods of TLC and HPLC can be used to detect the quality of L. cuneata.

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YANG Qing

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LI Yan-fang1

XIA Quan2

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Related Institution

Sixth Hospital Affiliated to Xinjiang Medical University

Tianjin University of Chinese Medicine

Xiyuan Hospital， China Academy of Chinese Medical Sciences

Tianjin University of Traditional Chinese Medicine

College of Traditional Chinese Medicine（TCM），Shandong University of TCM

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