Objective: To investigate the effects of different formulated Qiwei Baizhu San on the mRNA expression of interleukin-4 (IL-4)

IL-10 and interferon-α (IFN-α) in intestinal mucosal epithelia of mice with intestinal flora alteration. Method: Ceftriaxone

lincomycin hydrochloride and folium sennae were administered to mice by gavage to establish intestinal flora alteration type diarrhea models. After modeling

the mice were randomly divided into model group (MG

referred to as natural recovery group ZHG)

Qiwei Baizhu decoction group (QJG

6 g·kg-1)

Qiwei Baizhu drops group (QDG

1.36 g·kg-1)

Qiwei Baizhu extract group (QTG

0.27 g·kg-1)

and normal group (NG). Mice in MG (ZFG) and NG were given with distilled water by ig administration

and the mice in rest treatment groups were given with appropriate dosage of the drugs by ig administration. At days 7

9

11 and 13 of modeling

intestine was taken from 10 mice in each group for RT-PCR analysis of IL-4

IL-10 and IFN-α mRNA in intestinal mucosal epithelia of the mice. Result: Expressions of IL-4 mRNA and IL-10 mRNA in QJG QTG and QDG were higher than those in MG (P<0.05) at different treatment time. Expressions of IFN-α mRNA in NG

QJG

QTG and QDG were significantly lower than those in MG (P<0.05). At the different treatment time

IFN-α mRNA expression in intestinal mucosal epithelia of mice in QJG

QTG and QDG was gradually decreased (P<0.05). Conclusion: Different formulations of Qiwei Baizhu San can attenuate the intestinal inflammation

promote repair of damaged mucosa tissues by up-regulating the expressions of IL-4 and IL-10

and down-regulating the expression of IFN-α in intestinal mucosal epithelia. In addition

Qiwei Baizhu drops and Qiwei Baizhu extract are superior to Qiwei Baizhu decoction in regulating immune functions.