Objective: To observe the mechanism of Zaizao Wan Sailong bone replacement of leopard bone against cerebral ischemia

and provide experimental pharmacology data for replacement rationality. Method: The decapitation ischemia experiment and bilateral carotid artery ligation were used to establish mice models and observe the effect of Sailong bone Zaizao Wan (hereinafter referred to as Sailong bone Zaizao Wan) on acute cerebral ischemia in mice. Middle cerebral artery occlusion (MCAO) cerebral ischemic models were induced by using suture-occluded method. The effect of Sailong bone Zaizao Wan on infract area

neurological scores

malondialdehyde (MDA)

superoxide dismutase (SOD) and nitric oxide (NO) levels in serum were observed. 'Collagen-Adr' was used to induce thrombosis mice models and observe hemiplegia formation rate and mortality. Acute blood stasis rat models were used to observe hemorheology and platelet aggregation rate

and evaluate the effect of Sailong bone Zaizao Wan on activating blood circulation and dissipating blood stasis. Result: Sailong bone Zaizao Wan can increase breathing time and frequency of mice with acute cerebral ischemia after decollation

with no significant difference with Zaizao Wan group;and can significantly prolong the survival time of bilateral carotid artery ligation in mice with acute cerebral ischemia

with no significant difference with Zaizao Wan group. Sailong bone Zaizao Wan could significantly decrease the infarct size in MCAO rats

and 4

24 hours after ischemia

neurological function scores were decreased. Various treatment groups could significantly reduce serum MDA level in model rats;low dose group could improve serum SOD activity in MCAO model rats;various dose groups could significantly increase serum NO level in rats and the effect was better than Zaizao Wan group. Sailong bone Zaizao Wan could reduce the mortality within 5 min induced by tail intravenous injection of "collagen-Adr" thrombus formation

and increase the hemiplegia recovery rate within 15 min. It could decrease the whole blood flow shear rate and reduce ADP-induced platelet aggregation rate in blood stasis model rats. Conclusion: Sailong bone Zaizao Wan has protective effect on cerebral ischemia and its mechanisms may be related to its antioxidant capacity and improvement of hemorrheology.