Effect of Compound  on Expressions of Metabolic Genes in Rat Livers

GENG Shan; FENG Zhe; YUAN Cheng-chen; LU Wei; ZHU Ya-mei; ZHOU Xue-ping; ZHOU Ling-ling

doi:10.13422/j.cnki.syfjx.2016060140

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Effect of Compound on Expressions of Metabolic Genes in Rat Livers

更新时间：2024-01-04

- Effect of Compound on Expressions of Metabolic Genes in Rat Livers
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 22, Issue 6, Pages: 140-144(2016)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2016060140
  CLC： R285.5
- Published：2016
- 稿件说明：
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GENG Shan, FENG Zhe, YUAN Cheng-chen, et al. Effect of Compound on Expressions of Metabolic Genes in Rat Livers[J]. Chinese Journal of Experimental Traditional Medical Formulae, 2016, 22(6): 140-144.
DOI：

GENG Shan, FENG Zhe, YUAN Cheng-chen, et al. Effect of Compound on Expressions of Metabolic Genes in Rat Livers[J]. Chinese Journal of Experimental Traditional Medical Formulae, 2016, 22(6): 140-144. DOI： 10.13422/j.cnki.syfjx.2016060140.

摘要

目的: 从调控代谢酶角度探讨雷公藤复方配伍减轻雷公藤肝脏毒性的作用及分子机制。方法: SD大鼠给药1个月筛选雷公藤的肝毒性剂量;雷公藤单药和复方分别ig给药大鼠1个月

基因芯片检测大鼠肝脏基因表达谱

实时定量聚合酶链式反应(PCR)技术检测核受体组成型雄甾烷受体(CAR)

孕烷X受体(PXR)

过氧化物酶体增殖剂激活受体(PPAR)

芳香烃受体(AhR)的表达。结果: 雷公藤肝毒性剂量为15.6 g生药/kg;基因芯片结果显示

雷公藤可抑制细胞色素P450酶(CYP)2E1

CYP8B1

CYP2B等表达

诱导CYP7A1的表达。雷公藤复方可诱导代谢酶CYP3A4

CYP2E1

CYP8B1

CYP2B等表达。实时定量PCR结果显示

雷公藤可抑制核受体CAR

PXR

PPAR基因表达

雷公藤复方可改善雷公藤对核受体的抑制作用。结论: 雷公藤复方配伍可影响核受体

调控代谢酶的表达

这可能进而调节内外源代谢过程

从而起到减毒作用。

Abstract

Objective: To study the effect and mechanism of compound Tripterygium wilfordii(TW) on attenuating hepatotoxicity through regulation of metabolic enzymes. Method: Sprague dawley (SD) rats were intragastrically administrated with different doses of TW for one month to screen the dose of hepatotoxicity. TW single drug and compound TW were intragastrically administrated for one month respectively. Gene expression changes in rat liver were detected using gene chip technique

and expressions of constitutive androstane receptor (CAR)

pregnane X receptor (PXR)

peroxisome proliferators-activated receptors (PPARs) and aryl hydrocarbon receptor (AhR) were detected using real-time quantitative PCR. Result: Hepatotoxicity dose was 15.6 g·kg-1 of raw TW. Gene chip results showed that TW could inhibit expressions of cytocharome P450 enzymes(CYP) 2E1

CYP8B1 and CYP2B

and induce expression of CYP7A1.Compound TW could induce expressions of CYP3A4

CYP2E1

CYP8B1

and CYP2B. Real-time quantitative PCR results showed that TW could inhibit expressions of nuclear receptors CAR

PXR and PPAR

and Compound TW could improve its inhibitory effect on nuclear receptors. Conclusion: Compound TW preparations can affect expressions of nuclear receptors and metabolic enzymes

which may regulate endogenous and exogenous metabolic processes and thereby attenuate hepatotoxicity.

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