YANG Hong-mei, CAO Lei, SHAN Li-fang, et al. Optimization of Extraction Technology of Shuanghuang Erxian Granules by Orthogonal Test[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(7): 28-30.
DOI:
YANG Hong-mei, CAO Lei, SHAN Li-fang, et al. Optimization of Extraction Technology of Shuanghuang Erxian Granules by Orthogonal Test[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(7): 28-30. DOI: 10.13422/j.cnki.syfjx.2016070028.
Optimization of Extraction Technology of Shuanghuang Erxian Granules by Orthogonal Test
Objective: To optimize extraction technology of Shuanghuang Erxian granules. Method: HPLC was employed to determine contents of astragaloside Ⅳ and complanatuside in Shuanghuang Erxian granules with mobile phases of acetonitrile-water (35 : 65) and acetonitrile-0.2% phosphoric acid solution (21 : 79)
respectively.Taking contents of astragaloside Ⅳ and complanatuside
yield of dry extract as comprehensive evaluation index
orthogonal test was used to investigate effects of water amount
extracting time and times on extraction process of Shuanghuang Erxian granules. Result: Optimum extraction condition was as follows:extracted twice with 8 times the amount of water
1 hour for per time.Extracting amounts of astragaloside Ⅳ and complanatuside were 1.084
1.343 mg · g-1
yield of dry extract was 28.48%
extraction time had a significant effect on extraction technology of Shuanghuang Erxian granules. Conclusion: This optimized extraction condition is stable and feasible
it can provide guidance for industrial production of Shuanghuang Erxian granules.
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