Objective: To study the chemical constituents of Adenophorae Radix. Method: The compounds were isolated and purified by silica gel column

sephadex LH-20 column chromatography. Their structures were identified by physicochemical properties and spectral data. Result: Thirteen compounds have been isolated from the petroleum ether

ethyl acetate and n-butanol fractions. They were respectively identified as α-amyrin acetate (1)

taraxerone (2)

lupenone (3)

β-sitosterol (4)

lupeol acetate (5)

sessilifolic acid 3-O-isovalerate (6)

stigmastane (7)

n-pentadecanoic acid (8)

bis(2-ethylhexyl) phthalatel (9)

wogonoside (10)

guaiacol β-D-glucopyranoside (11)

5-hydroxymethyl furfural (12)

baicalin (13). Conclusion: Compound 1

8

10

11

13 were obtained from the Campanulaceae for the first time

compound 9

12 were obtained from the Adenophora for the first time

and compound 6 was obtained from this plant for the first time.