Simultaneous Determination of 8 Saponins in Ginseng Medicinal Fungal Substance by HPLC

ZHU Ying-ying; LIU Xin; JIA Ai-ling; WANG Lin; QIU Zhi-dong; ZHU Kai

doi:10.13422/j.cnki.syfjx.2016070096

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Simultaneous Determination of 8 Saponins in Ginseng Medicinal Fungal Substance by HPLC

更新时间：2024-01-04

- Simultaneous Determination of 8 Saponins in Ginseng Medicinal Fungal Substance by HPLC
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 22, Issue 7, Pages: 96-99(2016)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2016070096
  CLC： O657.72;R284.1
- Published：2016
- 稿件说明：
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ZHU Ying-ying, LIU Xin, JIA Ai-ling, et al. Simultaneous Determination of 8 Saponins in Ginseng Medicinal Fungal Substance by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(7): 96-99.
DOI：

ZHU Ying-ying, LIU Xin, JIA Ai-ling, et al. Simultaneous Determination of 8 Saponins in Ginseng Medicinal Fungal Substance by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(7): 96-99. DOI： 10.13422/j.cnki.syfjx.2016070096.

摘要

目的: 建立一种可以同时测定人参药性菌质中8种人参皂苷(人参皂苷Rg1

人参皂苷Re

人参皂苷Rf

人参皂苷Rh1

人参皂苷Rb1

人参皂苷Ro

人参皂苷Rb2

人参皂苷Rd)含量的HPLC方法。方法: 采用高效液相法

Inertsil ODS-SP色谱柱(4.6 mm×250 mm

5 μm)

流动相乙腈-0.4%磷酸水梯度洗脱

流速1 mL ·min-1

检测波长203 nm

柱温30 ℃

测定人参药性菌质8种皂苷的含量。结果: 8种单体皂苷在100 min内可良好分离

线性关系良好(R2>0.999 7)

平均加样回收率分别为100.15%

101.54%

101.87%

100.88%

102.10%

102.84%

101.38%

101.23%

RSD分别为1.6%

2.3%

1.9%

2.1%

1.5%

0.8%

2.7%

1.6%。结论: 该研究提供了一种能方便、简单、准确、重复性好的HPLC分析人参药性菌质中8种人参皂苷含量的方法

可为人参药性菌质成分转化研究的质量标准制定提供科学依据。

Abstract

Objective: To establish an HPLC method for simultaneous determination of ginsenoside Rg1

ginsenoside Re

ginsenoside Rf

ginsenoside Rh1

ginsenoside Rb1

ginsenoside Ro

ginsenoside Rb2

and ginsenoside Rd in ginseng medicinal fungal substance. Method: High performance liquid chromatography was carried out on Inertsil ODS-SP chromatographic column (4.6 mm×250 mm

5 μm) with acetonitrile-0.4% phosphate as the mobile phase for gradient elution. The flow rate was 1 mL · min-1

and detection wavelength was 203 nm. The column temperature was at 30 ℃. Result: Eight kinds of monomer saponins could be separated well in 100 min

with good linear relationship (R2>0.999 7).The average recovery rate was 100.15% (RSD 1.6%)

101.54% (RSD 2.3%)

101.87% (RSD 1.9%)

100.88% (RSD 2.1%)

102.10% (RSD 1.5%)

102.84% (RSD 0.8%)

101.38% (RSD 2.7%)

and 101.23% (RSD 1.6%). Conclusion: The HPLC method is accurate

reliable and reproducible to analyze 8 saponins in ginseng medicinal fungal substance

which can provide the scientific basis for formulating the quality standard of the research on composition transformation of the ginseng medicinal fungal substance.

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Related Institution

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