ZHANG Xue, ZHAO Bing-xiang, DONG Yan-hong, et al. Effects of Compatibility of Ginsenosides Rb and Aconitine on ATP Enzyme and Related Ion of Myocardial Cells in Newborn Rats[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(7): 112-115.
DOI:
ZHANG Xue, ZHAO Bing-xiang, DONG Yan-hong, et al. Effects of Compatibility of Ginsenosides Rb and Aconitine on ATP Enzyme and Related Ion of Myocardial Cells in Newborn Rats[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(7): 112-115. DOI: 10.13422/j.cnki.syfjx.2016070112.
Effects of Compatibility of Ginsenosides Rb and Aconitine on ATP Enzyme and Related Ion of Myocardial Cells in Newborn Rats
Objective: To study the effect of compatibility of aconitine and ginsenosides Rb1 on ATP enzyme and relatedion of myocardial cells. Method: Tryptic digestion and differential adherence method were used to culture myocardial cells in new born rats
and then these rats were divided into normal group
aconitine group
ginsenosides Rb1group
and aconitine and ginsenosides Rb1 compatibility (1 : 1
1 : 2
2 : 1) groups. After treatment for one hour
the activity of myocardial cells
ion concentration and ATP enzyme activity in myocardial cells were detected in various groups. Result: The 0.2% aconitine could reduce myocardial cells activity
reduce myocardial intracellular Mg2+ and K+ concentration
increase concentration of Ca2+ and Na+
and reduce Ca2+-ATPase
Na+-K+ ATPase and Ca2+-Mg2+-ATPase activities;compatibility of aconitine and ginsenosides Rb1 could increase myocardial intracellular Mg2+ and K+ concentration
reduce myocardial intracellular Ca2+ and Na+ concentration
and improve Ca2+-ATPase
Na+-K+-ATPase and Ca2+-Mg2+-ATPase activities. Conclusion: Ginsenosides Rb1 can reduce the aconitine toxicity on cardiomyocytes
and mechanism may be associated with regulating ATPase activity of myocardial cell membrane and related ion concentration in cells.
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Related Author
LI Haoran
CAO Ce
LI Lei
LIU Jianxun
Jian-ping YI
Jing DU
He MENG
Yu-ming CHI
Related Institution
Research Institute of Traditional Chinese Medicine (TCM) of Guangdong Pharmaceutical University
Beijing Key Laboratory of TCM Pharmacology,Xiyuan Hospital,China Academy of Chinese Medical Sciences,National Research Center for Clinical Medicine of Cardiovascular Diseases of TCM
Beijing Zhongyan Tongrentang Pharmaceutical Research and Development Co. Ltd.
Beijing Tongrentang Science and Technology Development Co. Ltd.
Department of Pharmacy,Jiangxi University of Traditional Chinese Medicine