DNA Molecular Identification of  Medicinal Plants in Solanaceae by ITS2 Barcode Sequence

WU Ya-nan; XU Liang; CHEN Liang; CAI Zhen-jiao; WANG Bing; ZHAO Rong; KANG Ting-guo

doi:10.13422/j.cnki.syfjx.2016080022

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DNA Molecular Identification of Medicinal Plants in Solanaceae by ITS2 Barcode Sequence

更新时间：2024-01-04

- DNA Molecular Identification of Medicinal Plants in Solanaceae by ITS2 Barcode Sequence
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 22, Issue 8, Pages: 22-27(2016)
- 作者机构：
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- DOI：10.13422/j.cnki.syfjx.2016080022
  CLC： R282.5
- Published：2016
- 稿件说明：
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WU Ya-nan, XU Liang, CHEN Liang, et al. DNA Molecular Identification of Medicinal Plants in Solanaceae by ITS2 Barcode Sequence[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(8): 22-27.
DOI：

WU Ya-nan, XU Liang, CHEN Liang, et al. DNA Molecular Identification of Medicinal Plants in Solanaceae by ITS2 Barcode Sequence[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(8): 22-27. DOI： 10.13422/j.cnki.syfjx.2016080022.

摘要

目的: 利用内转录间隔区2(internal transcribed spacer 2

ITS2)序列对茄科酸浆属植物进行分子鉴定。方法: 对酸浆属与散血丹属植物样本的ITS2序列进行逆转录-聚合酶链式反应(RT-PCR)扩增和测序。为扩大研究范围

从GenBank中下载了上述2个属植物样本的ITS2序列。所有序列经CodonCode Aligner V3.0.1软件拼接

DNAMAN软件比对分析

采用MEGA 5.10计算相关数据

基于K2P模型构建邻接聚类树。从ITS2数据库中获得所测样本及下载序列的ITS2二级结构信息

分析各样本间ITS2序列二级结构的差异。结果: 从邻接聚类树分析可知

所有样本被聚为两大支。酸浆属植物自成1支

散血丹属植物为1支

且除极个别样本外

2个属的各物种种内样本可各为1支

表现出单系性;且每2支之间的Bootstrap支持率均很高。结论: ITS2序列在近缘物种间的系统学研究、品种的鉴定方面具有较大的应用潜力

适用于酸浆属植物的分子鉴定。

Abstract

Objective: To identify Physalis plants in Solanaceae by internal transcribed spacer 2(ITS2) sequence. Method: ITS2 sequence of Physalis and Physaliastrum medicinal plant samples was amplified by RT-PCR and sequenced.In order to expand scope of research topic

ITS2 sequences of related species were downloaded from GenBank.All sequences were spliced by CodonCode Aligner V3.0.1

ITS2 sequences were compared and analyzed by DNAMAN software.Related data analysis and processing was performed by MEGA 5.10 software and neighbor-joining tree was constructed.ITS2 secondary structure information was predicted by ITS2 database and its differences of samples was analyzed. Result: All samples were clustered into two branches

Physaliastrum was clustered into a single branch and the same as Physalis.Except a few samples

all species of both genus were clustered into a branch where all intra-species samples were clustered to one branch respectively and obviously distinguished

which showed monophyletic.Bootstrap support rates between each two were very high. Conclusion: ITS2 sequences have great potential in terms of systematic research and species identification between related species.

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Related Institution

College of Pharmacy， Liaoning University of Traditional Chinese Medicine

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