Correlation Analysis Between Volatile Oil from  in Xinjiang Ethnic Medicine and Its Genetic Diversity

TANG Dai-ping; SHENG Ping; MIAO Li-juan; WANG Sa

doi:10.13422/j.cnki.syfjx.2016080041

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Correlation Analysis Between Volatile Oil from in Xinjiang Ethnic Medicine and Its Genetic Diversity

更新时间：2024-01-04

- Correlation Analysis Between Volatile Oil from in Xinjiang Ethnic Medicine and Its Genetic Diversity
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 22, Issue 8, Pages: 41-46(2016)
- 作者机构：
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- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2016080041
  CLC： R29
- Published：2016
- 稿件说明：
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TANG Dai-ping, SHENG Ping, MIAO Li-juan, et al. Correlation Analysis Between Volatile Oil from in Xinjiang Ethnic Medicine and Its Genetic Diversity[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(8): 41-46.
DOI：

TANG Dai-ping, SHENG Ping, MIAO Li-juan, et al. Correlation Analysis Between Volatile Oil from in Xinjiang Ethnic Medicine and Its Genetic Diversity[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(8): 41-46. DOI： 10.13422/j.cnki.syfjx.2016080041.

摘要

目的: 通过对多伞阿魏挥发油成分与其遗传多样性相关性研究

为多伞阿魏种质资源与品质评价提供重要科学依据。方法: 测定不同产地多伞阿魏挥发油成分含量

采用ISSR分子标记技术和聚类分析对来自省内8个县的176份多伞阿魏样品的遗传多样性进行研究

运用灰色关联法分析多伞阿魏遗传多样性对挥发油成分含量的影响。结果: 用所筛选的15条引物对多伞阿魏样品进行ISSR分析

共扩增出126条清晰的条带

其中多态性位点93个。8个居群中遗传多样性水平最高的是裕民县[多态性位点百分率(PPB)=29.37%

Nei's多样性指数(H)=0.1104

Shannon's信息指数(I)=0.1643)]

最低的是青河县(PPB=19.05%

H=0.0705

I=0.1054)。其中沙湾县与富蕴县的多伞阿魏居群间遗传距离最大(0.2199)

两者的遗传一致度最低(0.8026)

表明两者之间的亲缘关系最远

遗传差异较大。由H

PPB计算的多伞阿魏居群间的遗传多样性与人参新萜醇、龙脑、γ-桉叶油醇及挥发油成分总含量关联性较大。结论: 多伞阿魏居群间的遗传变异为63.08%

居群内的遗传变异为36.92%

多伞阿魏地理分布与遗传多样性具有显著关系

多伞阿魏3个遗传多样性因子中的Nei's多样性指数对多伞阿魏挥发性成分含量有着较大的影响。

Abstract

Objective: To provide an important scientific basis for Ferula ferulaeoides germplasm resources and quality evaluation through the study on correlation between the volatile oil and the genetic diversity of F. ferulaeoides. Method: The content of volatile oil of F. ferulaeoide from different origins was determined

and the genetic diversity of 176 samples from 8 counties in Xinjiang was studied with inter-simple sequence repeat(ISSR) molecular marker technique

and then the grey relational analysis (GRA) was used to analyze the effect of genetic diversity on the volatile oil content of F. ferulaeoides. Result: The ISSR analysis of the F. ferulaeoides samples was carried out by using 15 primers

a total of 126 clear bands were amplified and 93 of them were polymorphic. The highest level of genetic diversity among the 8 populations was in Yumin County[percentage of polymorphic bands (PPB)=29.37%

Shannon's information index (I)=0.1643

Nei's diversity index (H)=0.1104]

and the lowest level was in Qinghe County (PPB=19.05%

H=0.0705

I=0.1054). The genetic distance of F. ferulaeoides was greatest Shawan County and Fuyun County in Xinjiang (0.2199)

with the lowest genetic identity (0.8026)

indicating that these two areas had the farthest genetic relationship and great genetic differences. The genetic diversity of F. ferulaeoides calculated with H

I and PPB showed a strong correlation to the total contents of ginsenol

camphor

γ-eudesmol and volatile oils. Conclusion: The genetic variation was 63.08% in F. ferulaeoides between different populations

and 36.92% within the same population. The geographic distribution of F. ferulaeoides was significantly correlated with its genetic diversity

and the Nei's diversity index among the three factors had a greater impact on the volatile composition and content of F. ferulaeoide.

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