HPLC Fingerprint of Pien Tze Huang by Total Statistical Moment and Similarity Method

HU Chao; HE Hong; YANG Yan-tao; TANG Yu; ZHOU Yi-qun; HE Fu-yuan

doi:10.13422/j.cnki.syfjx.2016080053

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HPLC Fingerprint of Pien Tze Huang by Total Statistical Moment and Similarity Method

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- HPLC Fingerprint of Pien Tze Huang by Total Statistical Moment and Similarity Method
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 22, Issue 8, Pages: 53-57(2016)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2016080053
  CLC： O657.72;R286.0
- Published：2016
- 稿件说明：
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HU Chao, HE Hong, YANG Yan-tao, et al. HPLC Fingerprint of Pien Tze Huang by Total Statistical Moment and Similarity Method[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(8): 53-57.
DOI：

HU Chao, HE Hong, YANG Yan-tao, et al. HPLC Fingerprint of Pien Tze Huang by Total Statistical Moment and Similarity Method[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(8): 53-57. DOI： 10.13422/j.cnki.syfjx.2016080053.

摘要

目的: 对片仔癀HPLC指纹图谱总量统计矩及其相似度进行研究

进一步验证前期所创方法的关联性

并为片仔癀制剂质量控制提供依据。方法: 以三七皂苷R1

人参皂苷Rg1与Rb1

牛磺胆酸钠为对照

采用RP-HPLC建立片仔癀指纹图谱

并对指纹图谱总量统计矩及其相似度法的关联性进行分析。结果: 10批样品共有特征峰为14个

采用总量统计矩法计算其AUCT

相对AUCT

λT

相对λT

σT

相对σT的平均值分别为7.430×106 μV·s

1.002

22.540 min

0.972

5.391 min

1.065

RSD分别为5.0%

4.3%

1.0%

2.9%

2.9%;以生成的对照图谱为参考

采用总量统计矩相似度法分析得相似度平均值为0.943

RSD为1.9%;相关性分析得相对λT与相似度的相关系数为0.980

P<0.01

相对σT与相似度的相关系数为-0.683

P<0.05

均具有统计学意义。结论: 总量统计矩及其相似度法存在关联性

该法具有加合、偶联与统计性;所建立的片仔癀HPLC指纹图谱重复性好

准确度高

可用于质量控制。

Abstract

Objective: To study the fingerprints of Pien Tze Huang by total quantum statistical moment (TQSM) and total quantum statistical moment similarity (TQSMS) method

further verify the relevance between the previously established methods

and provide basis for the quality control of Pien Tze Huang. Method: With Notoginsenoside R1

ginsenoside Rg1

ginsenoside Rb1and sodium taurocholic acid as the control products

RP-HPLC was used to establish the chromatographic fingerprints of Pien Tze Huang

and the correlation between TQSM and TQSMS of the fingerprints was analyzed. Result: There were 14 common characteristic peaks for 10 batches of Pien Tze Huang samples. By using TQSM method

the average values of AUCT

relative AUCT

λT

relativeλT

σT

relative σTwere calculated as 7.430×106 μV·sec

1.002

22.540 min

0.972

5.391 min

1.065 and their RSD were 5.0%

4.3%

1.0%

2.9%

2.9% respectively. The average similarity was 0.943 and RSD was 1.9% compared with reference fingerprint by TQSMS. The correlation coefficient of relative λTand similarity was 0.980

P<0.01

and the correlation coefficient of relative σTand similarity was -0.683

P<0.05

with statistically significant difference. Conclusion: There was correlation between TQSM and TQSMS and this method was additive

couplable and statistical. Besides

the established chromatographic fingerprint was highly precise and reproducible

thus can be applied in the quality control of Pien Tze Huang.

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Related Institution

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