Objective: To study the chemical constituents from the aqueous extract of the fruits of Euodia rutaecarpa. Method: Compounds were isolated and purified by macroporous resin

silica gel

MCI resin

Sephadex LH-20

ODS column chromatography

and semi-preparative HPLC. Their structures were identified on the basis of physicochemical properties and spectral data. Result: Eleven compounds (including five phenolic acids and their glycosides

four flavonoids

one limonin glucoside and one alkaloid) were isolated from the aqueous extract of the fruits of E. rutaecarpa and identified as chlorogenic acid (1)

5-O-caffeoylquinic acid (2)

4-O-caffeoylquinic acid (3)

coniferin (4)

caffeic acid (5)

quercetin 3-O-α-D-arabinopyranoside (6)

hyperin (7)

rutin (8)

quercetin (9)

limonin diosphenol 17-β-D-glucopyranoside (10)

dehydroevodiamine (11). Conclusion: Compounds 2~4 and 6 were isolated from this genus for the first time. As confirmed by two-dimensional nuclear magnetic spectrum

the 1H-NMR spectral data of compound 10 was totally reported for the first time. In addition

by ultra high performance liquid chromatography-mass spectrometry (UPLC-MS)

chlorogenic acid (1)

5-O-caffeoylquinic acid (2) and 4-O-caffeoylquinic acid (3) were identified from E. rutaecarpa var. officinalis and E. rutaecarpa var. bodinieri for the first time

whereas

hyperin (7) was identified from E. rutaecarpa var. officinalis.