ZHOU Song-gui, LI Xin, LI Zhong-gai, et al. Effect of Process Improvement of Jinying Huanggui Decoction on Content of Total Polysaccharides[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(8): 65-68.
DOI:
ZHOU Song-gui, LI Xin, LI Zhong-gai, et al. Effect of Process Improvement of Jinying Huanggui Decoction on Content of Total Polysaccharides[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(8): 65-68. DOI: 10.13422/j.cnki.syfjx.2016080065.
Effect of Process Improvement of Jinying Huanggui Decoction on Content of Total Polysaccharides
Objective: To improve preparation process of Jinying Huanggui decoction for increasing the content of total polysaccharides in this preparation. Method: Step of petroleum ether extraction in preparation process of Jinying Huanggui decoction had been deleted
changed 70%
75% and 80% alcohol precipitation step to 80% alcohol precipitation by one step.Monosaccharide composition in total polysaccharides from Jinying Huanggui decoction was analyzed by GC-MS.Comparison of the content of total polysaccharides before and after process improvement was determined by phenol-sulfuric acid colorimetry. Result: Monosaccharide composition in total polysaccharides was composed of rhamnose
arabinose
xylose
mannose
glucose and galactose with molar ratio of 0.08:0.08:0.02:0.03:1.00:0.04;after process improvement
this molar ratio was 0.05:0.07:0.02:0.05:1.00:0.08.Before and after process improvement
average concentrations of total polysaccharides in Jinying Huanggui decoction were 11.52
22.69 g·L-1
respectively. Conclusion: This method is simple
accurate and reproducible
it can be used for determination of total polysaccharides of Jinying Huanggui decoction.Process improvement of this preparation is in favor of increasing of total polysaccharides content.
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Related Author
WANG Rui-hai
YE Ying
XU Jing
MIAO Qing
BAI Dong
LIU Li-mei
WANG Zhenxing
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Related Institution
Henan Collaborative Innovation Center of Research and Development on the Whole Industry Chain of Yuyao,Henan Research Center for Special Processing Technology of Chinese Medicine,Henan University of Chinese Medicine
Institute of Chinese Material Medica,China Academy of Chinese Medical Sciences
Affiliated Hospital of Nanjing University of Chinese Medicine
Engineering Center for Standardization of Chinese Medicine Processing Under State Ministry of Education, Nanjing University of Chinese Medicine