Extraction of Panax Chinese Medicines and Their Anti-cancer Effects on HEY Ovarian Cancer Cells

LIU Jian-lin; WU Qiu-shuang; WANG Yi-tao; ZHANG Qing-wen; LU Jin-jian

doi:10.13422/j.cnki.syfjx.2016080105

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Extraction of Panax Chinese Medicines and Their Anti-cancer Effects on HEY Ovarian Cancer Cells

更新时间：2024-01-04

- Extraction of Panax Chinese Medicines and Their Anti-cancer Effects on HEY Ovarian Cancer Cells
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 22, Issue 8, Pages: 105-110(2016)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2016080105
  CLC： R284.2;R285.5
- Published：2016
- 稿件说明：
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LIU Jian-lin, WU Qiu-shuang, WANG Yi-tao, et al. Extraction of Panax Chinese Medicines and Their Anti-cancer Effects on HEY Ovarian Cancer Cells[J]. Chinese Journal of Experimental Traditional Medical Formulae, 2016, 22(8): 105-110.
DOI：

LIU Jian-lin, WU Qiu-shuang, WANG Yi-tao, et al. Extraction of Panax Chinese Medicines and Their Anti-cancer Effects on HEY Ovarian Cancer Cells[J]. Chinese Journal of Experimental Traditional Medical Formulae, 2016, 22(8): 105-110. DOI： 10.13422/j.cnki.syfjx.2016080105.

摘要

目的: 从人参、西洋参、竹节参、三七这4个人参属中药中提取成分

对其抗卵巢癌活性进行筛选、比较。方法: 人参、西洋参、竹节参和三七分别用水和乙醇热回流提取制备相应的水提物和醇提物;上述药材70%乙醇水提取物经大孔树脂柱色谱分别制备得到总皂苷和相应的原人参二醇型皂苷和原人参三醇型皂苷部位。用噻唑蓝(MTT)法对这些提取物和部位在卵巢癌HEY细胞进行活性筛选

并用碘化丙啶(PI)染色检测提取物对细胞周期分布的影响

进一步用免疫印迹法(Western blot)检测了其对细胞周期蛋白表达的影响。划痕实验检测了提取物对细胞迁移能力的影响。结果: 各提取物都表现出不同程度地抑制卵巢癌HEY细胞增殖活性

其中西洋原人参二醇型皂苷(PDSPQ)、竹节参总皂苷(TSPJ)、人参醇提物(EEPG)、人参总皂苷(TSPG)、人参原人参二醇型皂苷(PDSPG)对HEY细胞的增殖抑制比较明显

抑制率分别为45.59%

45.78%

50.48%

46.98%

64.36%

PDSPQ

TSPG

PDSPG可以不同程度的下调周期蛋白依赖性激酶(CDK)4

CDK6

细胞周期蛋白D1(cyclin D1)的表达

但对细胞迁移能力没有明显影响。结论: 含有原人参二醇型皂苷的组分的抗卵巢癌HEY细胞活性比其他部位更明显

其机制可能与下调细胞周期相关蛋白CDK4

CDK6

cyclin D1的表达有关。

Abstract

Objective: To extract effective components from Panax ginseng

P. quinquefolium

P. japonicas and P. notoginseng

and compare their inhibitory activities against ovarian cancer HEY cells. Method: Water and ethanol extracts of P. ginseng

P. quinquefolium

P. japonicas and P. notoginseng were prepared by reflux extraction with water and ethanol. Total saponins and their panaxadiol saponin (PDS) and panaxtrol saponin (PTS) fractions were prepared from 70% ethanol water extracts of above herbs using macroporous resin chromatography. 3-(4

5-dimethyl-2-thiazolyl)-2

5-diphenyl-2-H-tetrazolium bromide (MTT) assay was used to detect the activities of these extracts and fractions against HEY ovarian cancer cells. propidium iodide(PI) staining was used to detect the effect of extracts on cell cycle distribution

and western blot was performed to detect their effect on expression of cell cycle related proteins. In addition

scratch assay was conducted to detect the effect of extracts on cell migration. Result: All the indicated extracts showed varied activities against HEY cells. Among them

panaxadiol saponin of P. quinquefolium (PDSPQ)

total saponin of P. japonicas (TSPJ)

ethanol extract of P. ginseng (EEPG)

total saponin of P. ginseng (TSPG)

and panaxadiol saponin of of P. ginseng(PDSPG) showed relative higher inhibitory rate against HEY cells

with 45.59%

45.78%

50.48%

46.98% and 64.36%

respectively. Further

PDSPQ

TSPG

and PDSPG could down-regulate the expressions of CDK4

CDK6

and cyclin D1 to different degrees

but had no significant effect on the migration of HEY cells. Conclusion: Fractions with PDS were more effective on inhibiting HEY cells growth

and the mechanism may be associated with down-regulating the protein expressions of CDK4

CDK6

and cyclin D1.

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