Objective: To investigate the effect of Notoginseng Radix et Rhizoma saponins

salidroside and effective components of Astragali Radix in mobilizing bone marrow mesenchymal stem cells in the rat model of myocardial infarction. Method: The SD rat model of myocardial infarction was established by Olivette method. The successfully modeled rats were randomly divided into:Huoxue group

Yiqi group

Yiqi and Huoxue group

model group and normal group

and then subdivided according to time points at 1

3

7

14

28 d. Huoxue group was given 8 mg·kg-1 of Xuesaitong soft capsule. Yiqi group were treated with 5 mg·kg-1 of Huangqi granule. Yiqi and Huoxue group was given 7 mg·kg-1 of Nuodikang capsule. Model group was given normal saline. Normal group received no drug. Normal group and model group were administered with equivalent normal saline by gavage once a day for 28 days. The positive cells percentage of CD54

CD106

CD105

CD117 in peripheral blood and bone marrow were detected by flow cytometry at different time points

and ELISA method was used to detect the expression of stem cell factor (SCF) in peripheral blood and bone marrow at different time points. Result: SCF level and positive cells percentage of CD105

CD117 content increased in model group since the first day

reached the peak on the 14th day

and then decreased gradually. But the increasing trend was superior in Huoxue group

Yiqi group

Yiqi and Huoxue group (P<0.05)

particularly Huoxue group was the best. The prescription of promoting blood circulation to remove blood stasis showed an obvious mobilizing effect. Positive cells percentage of CD54

CD106 in peripheral blood and bone marrow in model group reached the peak on the first day

and then decreased gradually

but Huoxue group showed a superior decreasing trend in CD54

CD106 than model group in peripheral blood (P<0.05). Compared with model group

CD54 and CD106 in bone marrow had no significant change. Conclusion: Notoginseng Radix et Rhizoma saponins

salidroside and effective components of Astragali Radix can mobilize bone marrow mesenchymal stem cells

and the mobilizing effect of the prescription of promoting blood circulation to remove blood stasis is the most obvious.