Objective: To observe the comprehensive regulating effects and mechanism of Yiqi Huoxue recipe on the p38 mitogen-activated protein kinases(p38MAPK) signal pathway and impaired endothelial function of diabetic rats. Method: High fat and high glucose diet and streptozotocin (streptozotocin

STZ) were used to establish the model of diabetic rats. The rats were randomly divided into 7 groups:normal group

model group

Danzhi Jiangtang capsules high dose group

middle dose group and low dose group (1.08

0.72

0.54 g·kg-1·d-1)

pioglitazone hydrochloride capsules group(10 mg·kg-1·d-1)

Danzhi Jiangtang capsules+pioglitazone hHydrochloride capsules group (1.08 g·kg-1·d-1+10 mg·kg-1·d-1). The diabetic model rats were given with corresponding dose medicine by intragastric administration

once a day

for 8 consecutive weeks. Blood samples were collected from abdominal aorta for detection. Western blot assay was used to detect p38MAPK

mitogen activated protein kinase kinasse3/6(MKK3/6)

cAMP response element-bingding protein1(CREB1) and mitogen-activated protein kinase phosphatase-1(MKP-1) protein expressions in endothelial cells. qPCR was used to detect the mRNA expression of monocyte chmotactic protein 1(MCP-1) and vascular endothelial cells Resistin in blood vessel endothelium. Result: Compared with the normal group

p38MAPK

MEK3/6

CREB1 protein expression and MCP-1

vascular endothelial cells Rresistin mRNA expression were significantly increased in the model group (P<0.01)

while MKP-1 protein expression was significantly reduced (P<0.01). Compared with the model group

p38MAPK

MEK3/6

CREB1 protein expression and MCP-1

vascular endothelial cells Resistin mRNA expression were significantly reduced in various treatment groups

while MKP-1 protein expression was significantly increased

with statistically significant difference (P<0.05). Conclusion: Yiqi Huoxue recipe can reduce the vascular endothelium injury by regulating the expression of p38MAPK signal transduction protein blood vessels of diabetic rats.