LI Xiao-wen, TONG Ling, LI Dong-xiang, et al. Research and Application of Zebrafish Neuronal Injury Model on Quality Control of Yangxue Qingnao Granules[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(9): 1-4.
DOI:
LI Xiao-wen, TONG Ling, LI Dong-xiang, et al. Research and Application of Zebrafish Neuronal Injury Model on Quality Control of Yangxue Qingnao Granules[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(9): 1-4. DOI: 10.13422/j.cnki.syfjx.2016090001.
Research and Application of Zebrafish Neuronal Injury Model on Quality Control of Yangxue Qingnao Granules
Objective: To investigate feasibility of application of zebrafish neuronal injury model on quality control for Yangxue Qingnao granules and to provide a new quality control method for this preparation. Method: Biological activity effect and stability of Yangxue Qingnao granules were quantitatively assessed in a zebrafish neuronal injury model which was established by mycophenolate mofetil (MMF). Result: Central nerve injury zebrafish model as a biological quality control standard for Yangxue Qingnao granules nerve cell protection exhibited excellent precision (intra-day RSD of 2.2%-3.7%
inter-day RSD of 1.2%)
repeatability (RSD of 8.4%) and stability (RSD of 4.9%).In the range of 0.1-1.0 g·L-1
there was a significant linear correlation;this method met requirements of biological activity determination of guiding principles. Conclusion: It can be turned out that methodology which established in dissertation is good at determining neuroprotective efficacy of Yangxue Qingnao granules
it is reliable
simple and repeatable
this method also provides a reference for development and improvement of bioassay of Chinese medicines.
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Key Laboratory of Zebrafish Modeling and Drug Screening for Human Diseases of Xiangyang City, Xiangyang No.1 People's Hospital, Hubei University of Medicine, Xiangyang
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