Chemical Constituents of Artemisiae Argyi Folium Extract and Its Anti-HBV Activity Screening

ZHAO Zhi-hong; WU Fang; ZHENG Li-yun; WANG Li-yang; HOU Ying-ying; GENG Nan; WANG Gui-fang

doi:10.13422/j.cnki.syfjx.2016090030

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Chemical Constituents of Artemisiae Argyi Folium Extract and Its Anti-HBV Activity Screening

更新时间：2024-01-04

- Chemical Constituents of Artemisiae Argyi Folium Extract and Its Anti-HBV Activity Screening
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 22, Issue 9, Pages: 30-34(2016)
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- DOI：10.13422/j.cnki.syfjx.2016090030
  CLC： R284.1
- Published：2016
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ZHAO Zhi-hong, WU Fang, ZHENG Li-yun, et al. Chemical Constituents of Artemisiae Argyi Folium Extract and Its Anti-HBV Activity Screening[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(9): 30-34.
DOI：

ZHAO Zhi-hong, WU Fang, ZHENG Li-yun, et al. Chemical Constituents of Artemisiae Argyi Folium Extract and Its Anti-HBV Activity Screening[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(9): 30-34. DOI： 10.13422/j.cnki.syfjx.2016090030.

摘要

目的: 研究菊科艾Artemisia argyi干燥叶的化学成分及抗乙型肝炎病毒(HBV)活性。方法: 采用硅胶柱色谱分离其化学成分

并根据理化性质、核磁共振波谱法、质谱法以及参考相关文献鉴定化合物结构

采用噻唑蓝(MTT)法测定化合物对HepG 2.2.15细胞的毒性以及酶联免疫(ELISA)法检测各组分对HepG 2.2.15细胞上清液中HBeAg和HBsAg分泌的影响。结果: 从艾叶乙酸乙酯部位分离鉴定了5个化合物

分别为豆甾醇(1)

4'-二羟基-6

3'-三甲氧基黄酮(3'-甲氧基蓟黄素)(2)

达玛二烯醇乙酸酯(3)

4'-三羟基-3'

8-二甲氧基黄酮(4)

十六烷酸丙酯(5)。结论: 化合物 2~4 为首次从艾叶中分离得到

其中3'-甲氧基蓟黄素对HBsAg和HBeAg有抑制作用

其对HBsAg的半数抑制浓度(IC50)为8.09 mg·L-1

对HBeAg的IC50小于2.5 mg·L-1。

Abstract

Objective: To study the chemical components of Artemisiae Argyi Folium and their anti-hepatitis B virus(HBV) activities. Method: The chemical components were isolated and purified by silica gel column chromatography

and their structures were identified according to their physicochemical properties

nuclear magnetic resonance spectroscopy (NMR)

mass-spectrography (MS) and literature analysis. Furthermore

the toxicity of these chemical components to HepG 2.2.15 cells was measured by MTT method and their effects on HBeAg and HBsAg secretion in HepG 2.2.15 cell supernatant were measured by ELISA method. Result: Five compounds were isolated from Artemisiae Argyi Folium and their structures were identified as stigmasterol(1)

4'-dyhydroxyl-6

3'-trimethoxyflavone (3'-methoxythistle flavin) (2)

dammaradienylacetate(3)

4'-trihydroxy-3'

8-dimethoxyflavone(4)

and hexadecanoic dihydro ypropyleste(5). Conclusion: Compounds 2-4 were isolated from Artemisiae Argyi Folium for the first time and 3'-methoxythistle flavin showed inhibitory effect on HBsAg and HBeAg lines with IC50values of 8.09 mg·L-1 and less than 2.5 mg·L-1respectively.

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