Simultaneous Determination of Six Flavonoids in Extract of Microctis Folium by HPLC

JIANG Jie-yi; LI Yang-xue; LI Su-mei; XU Ai-li

doi:10.13422/j.cnki.syfjx.2016090040

您当前的位置：

首页 >

文章列表页 >

Simultaneous Determination of Six Flavonoids in Extract of Microctis Folium by HPLC

更新时间：2024-01-04

- Simultaneous Determination of Six Flavonoids in Extract of Microctis Folium by HPLC
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 22, Issue 9, Pages: 40-43(2016)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2016090040
  CLC： O657.72;R284.1
- Published：2016
- 稿件说明：
移动端阅览
JIANG Jie-yi, LI Yang-xue, LI Su-mei, et al. Simultaneous Determination of Six Flavonoids in Extract of Microctis Folium by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(9): 40-43.
DOI：

JIANG Jie-yi, LI Yang-xue, LI Su-mei, et al. Simultaneous Determination of Six Flavonoids in Extract of Microctis Folium by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(9): 40-43. DOI： 10.13422/j.cnki.syfjx.2016090040.

摘要

目的: 建立布渣叶总黄酮提取物中表儿茶素、牡荆苷、异牡荆苷、山柰酚-3-O-芸香糖苷、异鼠李素-3-O-葡萄糖苷和水仙苷等6种黄酮类成的HPLC同时测定方法。方法: 采用HPLC法

Agilent HC-C18色谱柱(4.6 mm×250 mm

5 μm)

甲醇-0.05%磷酸水溶液为流动相进行梯度洗脱

检测波长288 nm

柱温35℃

流速0.8 mL·min-1。结果: 表儿茶素、牡荆苷、异牡荆苷、山柰酚-3-O-芸香糖苷、异鼠李素-3-O-葡萄糖苷和水仙苷分别在131.200~1 312.000

149.800~1 348.200

131.400~1 051.200

99.175~595.050

109.955~659.730

267.000~1 348.200 ng呈线性关系

平均回收率分别为98.13%

97.41%

96.93%

98.22%

97.37%

97.82%。结论: 该方法准确、可靠

重复性好

可用于布渣叶总黄酮提取物的质量评价与控制。

Abstract

Objective: To establish a HPLC method for simultaneous determinationof the content of epicatechin

vitexin

isovitexin

kaemferol-3-O-rutinoside

isorhamnetin-3-O-β-D-glucoside

and narcissi in extract of Microctis Folium. Method: The HPLC analysis was carried out on Agilent HC-C18column (4.6 mm×250 mm

5 μm) with amethanol-0.05% phosphoric acid solution as the mobile phase for gradient elution at a flow rate of 0.8 mL·min-1.The detection wavelength was 288 nm

and the column temperature was 35℃. Result: Good linear relationship were found in the the range of 131.200-1 312.000 ng for epicatechin

149.800-1 348.200 ng for vitexin

131.400-1 051.200 ng for isovitexin

99.175-595.050 ng for kaemferol-3-O-rutinoside

109.955-659.730 ng for isorhamnetin-3-O-β-D-glucoside

and 267.000-1 348.200 ng for narcissi. Their average recoveries were 98.13%

97.41%

96.93%

98.22%

97.37%

97.82%

respectively. Conclusion: The method is accurate

reliable

and can be used for the quality control and evaluationof total flavonoid extracts of Microctis Folium.

关键词

Keywords

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Study on Quality Evaluation of Hawthorn

Determination of Vitexin in Microcos Folium by HPLC

Simultaneous Determination of Three Flavonoids in Extract of by HPLC

HPLC Fingerprint of Bletillae Formosanae Rhizoma

HPLC Fingerprint of Different Polarity Fractions of Gardeniae Fructus with Different Fruit Shapes

Related Author

CHEN Cheng-yu

YANG Bin

ZHOU Jie

LUO Wen-hui

TAN Zhi-can

SUN Dong-mei

CHEN Xue-ting

Related Institution

College of Pharmaceutical Science, Yunnan University of Traditional Chinese Medicine

Henan University of Chinese Medicine

Zhongjing Wanxi Pharmaceutical Co. Ltd.

Hubei Institute for Food and Drug Control

Shanxi Renyuantang Pharmaceutical Co.,Ltd.

AI问答

Postal code：100700
Tel：010-84076882 Email：syfjx_2010@188.com
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备11006657号-10 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰