Simultaneous Determination of Six Compounds in  var.  by UPLC

CHANG An; WANG Wei-ning; HAO Yan-jun; YANG Yan-yun; XU Liang; KANG Ting-guo

doi:10.13422/j.cnki.syfjx.2016090048

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Simultaneous Determination of Six Compounds in var. by UPLC

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- Simultaneous Determination of Six Compounds in var. by UPLC
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 22, Issue 9, Pages: 48-51(2016)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2016090048
  CLC： O657.72;R284.1
- Published：2016
- 稿件说明：
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CHANG An, WANG Wei-ning, HAO Yan-jun, et al. Simultaneous Determination of Six Compounds in var. by UPLC[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(9): 48-51.
DOI：

CHANG An, WANG Wei-ning, HAO Yan-jun, et al. Simultaneous Determination of Six Compounds in var. by UPLC[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(9): 48-51. DOI： 10.13422/j.cnki.syfjx.2016090048.

摘要

目的: 建立超高效液相色谱法(UPLC)同时测定狭山野豌豆中6种成分(绿原酸、杨梅苷、金丝桃苷、异槲皮苷、山柰苷、槲皮苷)的含量测定方法。方法: 采用Eclipse Plus C18 RRHD色谱柱(2.1 mm×50 mm

1.8 μm)

以乙腈(B)-0.1%甲酸溶液(A)为流动相进行梯度洗脱(0~7 min

5%~10%B;7~11 min

10%~12%B;11~20 min

12%B;20~25 min

12%~19%B;25~30 min

19%~24%B)

流速0.2 mL·min-1

检测波长340 nm

柱温30℃。结果: 狭山野豌豆中6个成分在线性范围内呈现良好的线性关系

r> 0.999 9

回收率均在99.15%~101.98%

RSD 0.7%~1.7%。结论: 该方法操作简便、精确

稳定性重复性好

可用于同时测定狭山野豌豆中6种化学成分的含量

为狭山野豌豆的质量控制提供依据。

Abstract

Objective: To establish an UPLC method for simultaneously determining content of 6 major compounds (chlorogenic acid

myricitrin

hyperoside

isoquercitrin

kaempferitrin

quercitrin) in Vicia amoena var. angusta. Method: An Eclipse Plus C18 RRHD column (2.1 mm×50 mm

1.8 μm) was adopted

with acetonitrile (B)-0.1% formic acid aqueous solution (A) as mobile phase for gradient elution (0-7 min

5%-10%B;7-11 min

10%-12%B;11-20 min

12%B;20-25 min

12%-19%B;25-30 min

19%-24%B). The flow rate was 0.2 mL·min-1. The detection wavelength was set at 340 nm and the column temperature was at 30℃. Result: Six components of V. amoena var. angusta showed good linear relationship in the linear range(r> 0.999 9) and the average recoveries were between 99.15% to 101.98% with the RSD of 0.7%-1.7%. Conclusion: The developed method is convenient

rapid and accurate with high repeatability and stability

suitable for simultaneously determining contents of 6 compounds in V. amoena var. angusta and can provide basis for the quality control of V. amoena var. angusta.

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Related Institution

Pharmacy College of Liaoning University of Traditional Chinese Medicine

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Henan University of Chinese Medicine

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