Simultaneous Determination of Six Components in Extract of Modified Xiaoyao by UPLC

LI Ming-yue; ZHOU Li-hong; ZHANG Wen-sheng; ZHANG Shun-nan; YE Zheng-liang; LIU Wen-yuan

doi:10.13422/j.cnki.syfjx.2016090058

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Simultaneous Determination of Six Components in Extract of Modified Xiaoyao by UPLC

更新时间：2024-01-04

- Simultaneous Determination of Six Components in Extract of Modified Xiaoyao by UPLC
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 22, Issue 9, Pages: 58-62(2016)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2016090058
  CLC： O657.72;R284.1
- Published：2016
- 稿件说明：
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LI Ming-yue, ZHOU Li-hong, ZHANG Wen-sheng, et al. Simultaneous Determination of Six Components in Extract of Modified Xiaoyao by UPLC[J]. Chinese Journal of Experimental Traditional Medical Formulae, 2016, 22(9): 58-62.
DOI：

LI Ming-yue, ZHOU Li-hong, ZHANG Wen-sheng, et al. Simultaneous Determination of Six Components in Extract of Modified Xiaoyao by UPLC[J]. Chinese Journal of Experimental Traditional Medical Formulae, 2016, 22(9): 58-62. DOI： 10.13422/j.cnki.syfjx.2016090058.

摘要

目的: 建立同时测定加味逍遥提取物中6个指标性成分(栀子苷、芍药内酯苷、芍药苷、甘草苷、苯甲酰芍药苷、甘草酸)含量方法。方法: 采用超高效液相色谱法

以UPLCTM HSS T3色谱柱(2.1 mm×100 mm

1.8 μm)

乙腈-0.05%磷酸水溶液为流动相梯度洗脱

柱温30℃

体积流量0.4 mL·min-1

检测波长230 nm。结果: 6个指标性成分分离度良好

均在各自线性范围内线性关系良好

R2≥0.999 4;平均加样回收率为98.29%~101.67%

RSD均< 3.8%。结论: 该法准确、简便、分离度良好

可作为加味逍遥提取物的质量评价方法。

Abstract

Objective: To establish an UPLC method for the simultaneous determination of geniposide

albiflorin

paeoniflorin

liquiritin

benzoylpaeoniflorin

and glycyrrhizic acid in extract of modified Xiaoyao extract. Method: UPLC was performed on UPLCTM HSS T3 column (2.1 mm×100 mm

1.8 μm) with acetonitrile-0.05% phosphoric acid solution as the mobile phase for gradient elution. The detection wavelength was set at 230 nm. The flow rate was 0.4 mL·min-1 and column temperature was 30℃. Result: The six components were well separated and showed good linearity in their respective concentration ranges with R2≥0.999 4. The average recovery was 98.29%-101.67% with RSD less than 3.8%. Conclusion: The established method is simple and stable with satisfactory separation degree. It can be used for the quality control of modified Xiaoyao extract.

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