Objective: To investigate effect of total flavonoids from Fagopyrum tataricum on expression of nicotinamide adenine dinucleotidephosphateoxidase-4 (NOX4) in EA. hy926 cell induced by palmitic acid

and to discuss mechanism of total flavonoids from F. tataricum in signal transduction pathway of insulin resistance. Method: EA. hy 926 cells were divided into blank group

insulin resistance model group

total flavonoids of F. tataricum group (31.25

62.5

125 mg·L-1) and metformin group.Every groups were combined insulin with 50 nmol·L-1.Compared with the blank group

other groups were combined 600 μmol·L-1 palmitic acid.Total flavonoids of F. tataricum group was combined 125 mg·L-1 total flavonoids of F. tataricum.Metformin group was combined 2 mmol·L-1 metformin.The content of asymmetric dimethylarginine (ADMA) was tested by double antibody sandwich method.The content of NO was tested by nitrate reductase method.NOX4 protein expression levels were determined by Western blot. Result: Compared with model group

contents of ADMA in the middle and high dose groups of total flavonoids were significantly reduced

contents of NO were increased significantly.Compared with model group

expression levels of NOX4 protein were reduced significantly in high dose groups of total flavonoids. Conclusion: Total flavonoids from F. tataricum can promote synthesis of NO by inhibiting synthesis of NOX4 and ADMA in EA. hy926 cell induced by palmitic acid

then it may restrain insulin resistance.