Objective: To establish and compare the fingerprint of Moutan Cortex and wined Moutan Cortex by HPLC. Method: HPLC method was adopted on Luna C18 column (4.6 mm×250 mm

5 μm)

with acetonitrile-0.2% phosphoric acid solution as the mobile phase for gradient elution. The flow rate was 0.8 mL·min-1

and the column temperature was maintained at 25℃. The detection wavelength was 230 nm

and the injection volume was 10 μL. Result: There were 12 common peaks in HPLC fingerprint of Moutan Cortex and wined Moutan Cortex. The similarity between various samples and the automatically generated reference fingerprints reached above 0.90. There were little differences in fingerprint of Moutan Cortex before and after processing

but the peak areas of No. 3 peak and No. 6 peak were increased significantly in wined Moutan Cortex. Conclusion: The established method is accurate and reliable

and can provide experimental basis for further study on wined Moutan Cortex.